Application of Phyllanthus emblica extract in manufacturing pharmaceutical composition for repairing liver damage

Lai, Y.C. and Kuo, C.Y. and Yang, S.C. and Tu, C.T. and Cheng, H.C. (2022) Application of Phyllanthus emblica extract in manufacturing pharmaceutical composition for repairing liver damage. Food Research (Malaysia), 6. pp. 154-160. ISSN 2550-2166

The objective of this study was to prepare a medicinal formulation with the extract of Phyllanthus emblica to repair liver damage. The hepatocyte cell line Hep-G2 was used in the experiment. The experimental sample was prepared by adding 20,000 hepatocytes to each well of a 24-well Seahorse XF24 analyzer special cell culture plate and cultured for 24 hrs. Subsequently, the culture medium from each well was removed. The hepatocytes in the wells were processed according to the conditions of the experimental, control, or comparator group. The oxygen consumption of the hepatocytes in the experimental, control, and comparator groups in the wells of the cell culture plate was measured using the Seahorse XF24 analyzer bioenergy meter. Treating cells with hydrogen peroxide can simulate the intracellular oxidation of free radicals, permitting examination of the mitochondrial activity of cells under oxidative stress. The mitochondrial activity in the hepatocytes was maintained. The mitochondria produced a sufficient amount of adenosine triphosphate (ATP), allowing the hepatocytes to maintain their normal metabolic functions. Owing to the improved synthesis efficiency and capability of triphosphate required for cell damage repair, damaged hepatocytes were able to obtain adequate energy for repair. Thus, liver repair was accelerated, and it returned to its normal condition.

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Abstract

The objective of this study was to prepare a medicinal formulation with the extract of Phyllanthus emblica to repair liver damage. The hepatocyte cell line Hep-G2 was used in the experiment. The experimental sample was prepared by adding 20,000 hepatocytes to each well of a 24-well Seahorse XF24 analyzer special cell culture plate and cultured for 24 hrs. Subsequently, the culture medium from each well was removed. The hepatocytes in the wells were processed according to the conditions of the experimental, control, or comparator group. The oxygen consumption of the hepatocytes in the experimental, control, and comparator groups in the wells of the cell culture plate was measured using the Seahorse XF24 analyzer bioenergy meter. Treating cells with hydrogen peroxide can simulate the intracellular oxidation of free radicals, permitting examination of the mitochondrial activity of cells under oxidative stress. The mitochondrial activity in the hepatocytes was maintained. The mitochondria produced a sufficient amount of adenosine triphosphate (ATP), allowing the hepatocytes to maintain their normal metabolic functions. Owing to the improved synthesis efficiency and capability of triphosphate required for cell damage repair, damaged hepatocytes were able to obtain adequate energy for repair. Thus, liver repair was accelerated, and it returned to its normal condition.

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Item Type:	Article
AGROVOC Term:	Phyllanthus emblica
AGROVOC Term:	manufacturing
AGROVOC Term:	cell culture
AGROVOC Term:	hydrogen peroxide
AGROVOC Term:	oxidative stress
Geographical Term:	China
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	06 Feb 2026 07:33
Last Modified:	06 Feb 2026 07:33
URI:	http://webagris.upm.edu.my/id/eprint/3348

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