Optimization of medium for lipid production from Lipomyces maratuensis InaCC Y720 using statistical experiment design

Liya Audinah, . and Atit Kanti, . and Miftahul Ilmi, . (2024) Optimization of medium for lipid production from Lipomyces maratuensis InaCC Y720 using statistical experiment design. Pertanika Journal Tropical Agricultural Science (Malaysia), 47 (3). pp. 905-921. ISSN 1511-3701

Lipomyces maratuensis InaCC Y720 is a potential novel oleaginous yeast. Media-based production optimization has never been carried out using this strain. This study aims to define an optimized medium from 12 medium component factors, where the Taguchi method is used for screening significant factors of medium and the response surface methodology (RSM) is used to optimize the concentration of significant factors. According to Taguchi, glucose, yeast extract, and magnesium sulfate (MgSO₄) have a significant influence on lipid accumulation, with their concentrations maintained at optimal levels through RSM optimization. Conversely, potassium dihydrogen phosphate, sodium hydrogen phosphate, and calcium chloride inhibit lipid accumulation, and copper(II) sulfate has the least influence, categorizing them as eliminated factors. The RSM-optimized medium increased lipid content by 3.6-fold compared to the initial medium. Glucose and yeast extract showed a positive correlation with lipid accumulation, suggesting potential for further optimization, while the optimum concentration for MgSO4 was 0.15 g/L. This study is intended to serve as a reference for increasing lipid accumulation by L. maratuensis InaCC Y720.

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Abstract

Lipomyces maratuensis InaCC Y720 is a potential novel oleaginous yeast. Media-based production optimization has never been carried out using this strain. This study aims to define an optimized medium from 12 medium component factors, where the Taguchi method is used for screening significant factors of medium and the response surface methodology (RSM) is used to optimize the concentration of significant factors. According to Taguchi, glucose, yeast extract, and magnesium sulfate (MgSO₄) have a significant influence on lipid accumulation, with their concentrations maintained at optimal levels through RSM optimization. Conversely, potassium dihydrogen phosphate, sodium hydrogen phosphate, and calcium chloride inhibit lipid accumulation, and copper(II) sulfate has the least influence, categorizing them as eliminated factors. The RSM-optimized medium increased lipid content by 3.6-fold compared to the initial medium. Glucose and yeast extract showed a positive correlation with lipid accumulation, suggesting potential for further optimization, while the optimum concentration for MgSO4 was 0.15 g/L. This study is intended to serve as a reference for increasing lipid accumulation by L. maratuensis InaCC Y720.

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Item Type:	Article
AGROVOC Term:	lipids
AGROVOC Term:	glucose
AGROVOC Term:	yeast extracts
AGROVOC Term:	experimental design
AGROVOC Term:	statistical methods
Geographical Term:	Indonesia
Depositing User:	Ms. Azariah Hashim
Date Deposited:	26 Feb 2026 06:52
Last Modified:	26 Feb 2026 06:52
URI:	http://webagris.upm.edu.my/id/eprint/3389

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