Plant extracts as an alternative for antibiotics in saanen goats’ semen extender

Nur Hafizah M., . and Mashitah S. M., . and Siti Syamsiah I., . and Noor Izza M., . and Norzuraiti M. I., . (2024) Plant extracts as an alternative for antibiotics in saanen goats’ semen extender. Pertanika Journal Tropical Agricultural Science (Malaysia), 52 (2). pp. 49-58. ISSN 1511-3701

The widespread use of antibiotics contributes to the development of antibiotic resistance. Therefore, exploring suitable alternatives to antibiotics is crucial. This study aimed to determine the antibacterial effect of mulberry, roselle and aloe vera extracts on bacteria isolated from buck semen and their effectiveness as an alternative to antibiotics for semen extender. The antibacterial activity of plant extract against identified bacteria was determined by using disc diffusion method. Semen was added to the extender with mulberry, roselle or aloe vera extract and incubated at 37 °C for 3 h and cryopreserved for 24 h before the assessment of bacterial load and semen quality were investigated. Results shows that mulberry extract was the only plant extract that significantly inhibited both gram-negative and gram-positive bacteria, with greater inhibitory zone on gram positive bacteria (22.33 ± 1.12 mm) compare to Roselle (8.1 ± 1.08 mm) and Aloe vera (9.6 ± 1.05 mm) resulting in the lowest bacterial load (763 ± 4.57 CFU/ml) and improved sperm quality during the 3-hour incubation period. Whereas, aloe vera and roselle extracts were highly effective for decreasing bacterial contamination and protecting the sperm quality of post-thawed semen. The study concluded that, mulberry extract was a suitable alternative to commercial antibiotics for fresh semen extenders, while aloe vera and roselle were more recommended for cryopreservation purposes.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/52-2/Nur%20Hafizah...

Abstract

The widespread use of antibiotics contributes to the development of antibiotic resistance. Therefore, exploring suitable alternatives to antibiotics is crucial. This study aimed to determine the antibacterial effect of mulberry, roselle and aloe vera extracts on bacteria isolated from buck semen and their effectiveness as an alternative to antibiotics for semen extender. The antibacterial activity of plant extract against identified bacteria was determined by using disc diffusion method. Semen was added to the extender with mulberry, roselle or aloe vera extract and incubated at 37 °C for 3 h and cryopreserved for 24 h before the assessment of bacterial load and semen quality were investigated. Results shows that mulberry extract was the only plant extract that significantly inhibited both gram-negative and gram-positive bacteria, with greater inhibitory zone on gram positive bacteria (22.33 ± 1.12 mm) compare to Roselle (8.1 ± 1.08 mm) and Aloe vera (9.6 ± 1.05 mm) resulting in the lowest bacterial load (763 ± 4.57 CFU/ml) and improved sperm quality during the 3-hour incubation period. Whereas, aloe vera and roselle extracts were highly effective for decreasing bacterial contamination and protecting the sperm quality of post-thawed semen. The study concluded that, mulberry extract was a suitable alternative to commercial antibiotics for fresh semen extenders, while aloe vera and roselle were more recommended for cryopreservation purposes.

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Item Type:	Article
AGROVOC Term:	semen
AGROVOC Term:	plant extracts
AGROVOC Term:	goats
AGROVOC Term:	semen preservation
AGROVOC Term:	cryopreservation
AGROVOC Term:	roselle
AGROVOC Term:	Aloe vera
AGROVOC Term:	Bacteria
AGROVOC Term:	antibiotics
AGROVOC Term:	antibiotic resistance genes
Geographical Term:	Malaysia
Depositing User:	Ms. Azariah Hashim
Date Deposited:	23 Jul 2026 04:26
Last Modified:	23 Jul 2026 04:26
URI:	http://webagris.upm.edu.my/id/eprint/3543

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