Pineapple peel bromelain extract improves poultry in vitro protein digestibility

N.S. Zailani, . and Foo, H. L. and M. F. Abdul Aziz, . and Loh, T. C. and H. Akit, . (2024) Pineapple peel bromelain extract improves poultry in vitro protein digestibility. Malaysian Journal of Animal Science (Malaysia), 27 (2). pp. 53-64. ISSN 2520-2123

This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone and in combination with 0.02 M dithiothreitol (DTT), 0.02% sodium azide, and 0.02 M hylenediaminetetraacetic acid (EDTA). Subsequently, the effects of bromelain extracts on protein solubilization and digestibility using a poultry gut simulation model were evaluated. The findings indicated that bromelain extractions using deionized water (82.22 U/mg) and a combination of deionized water + sodium azide + EDTA (87.84 U/mg) exhibited the highest proteolytic activities compared to other extractants (P<0.05). Furthermore, pineapple peel bromelain extracts resulted in up to 24% increase in protein solubility and up to 6% increase in protein digestibility (P<0.05, respectively). Notably, bromelain extracted using deionized water (60.69 U/mg) proved to be the most effective in digesting protein in vitro.

Full text available from: Official URL: https://mjas.my/mjas-v2/rf/pages/journal/v27i2/4.%...

Abstract

This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone and in combination with 0.02 M dithiothreitol (DTT), 0.02% sodium azide, and 0.02 M hylenediaminetetraacetic acid (EDTA). Subsequently, the effects of bromelain extracts on protein solubilization and digestibility using a poultry gut simulation model were evaluated. The findings indicated that bromelain extractions using deionized water (82.22 U/mg) and a combination of deionized water + sodium azide + EDTA (87.84 U/mg) exhibited the highest proteolytic activities compared to other extractants (P<0.05). Furthermore, pineapple peel bromelain extracts resulted in up to 24% increase in protein solubility and up to 6% increase in protein digestibility (P<0.05, respectively). Notably, bromelain extracted using deionized water (60.69 U/mg) proved to be the most effective in digesting protein in vitro.

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Item Type:	Article
AGROVOC Term:	proteins
AGROVOC Term:	extraction
AGROVOC Term:	poultry
AGROVOC Term:	sodium azide
AGROVOC Term:	EDTA
AGROVOC Term:	in vitro
AGROVOC Term:	laboratory experimentation
Geographical Term:	Malaysia
Depositing User:	Ms. Azariah Hashim
Date Deposited:	29 Jul 2026 03:17
Last Modified:	29 Jul 2026 03:17
URI:	http://webagris.upm.edu.my/id/eprint/3557

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