Thin layer chromatography method for terpenoid and flavonoid detection in Dendrophthoe pentandra(L.) miq on Ficus religiosa L.

Djoko Santosa, . and Widyastuti, . and Ummi Rosyidah, . and Betha Silmia, . (2022) Thin layer chromatography method for terpenoid and flavonoid detection in Dendrophthoe pentandra(L.) miq on Ficus religiosa L. International Journal of Agriculture, Forestry and Plantation (Malaysia), 12. pp. 101-105. ISSN 2462-1757

Mistletoe has been acknowledged as medicinal plant for traditional treatment because it possesses beneficial compound which potentially aid various diseases. Despite its destructive nature, each species of mistletoes can exhibit different beneficial compounds. Exploratory research has been conducted for detection secondary metabolites of Dendrophthoe pentandra that grow on the Bodhi tree (Ficus religiosa L.) at Universitas Gadjah Mada, Yogyakarta, Indonesia. The aim of this research is to detect secondary metabolites which potentially for medicational purpose in D. pentandra using thin layer chromatography method. The mistletoes sample was obtained from the Bodhi tree, authentication of the species of mistletoes was carried out at the Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada. D. pentandra leaf was washed and dried in a dryer cabinet at 50o C, it was subsequently powdered. Amount of 5 g of leaf powder was macerated with 50 ml of 95% ethanol. Analysis of chemical compounds was carried out using the Thin Layer Chromatography (TLC) method with silica gel 60 F254 as a stationary phase and a mobile phase suitable for terpenoids and flavonoids compounds. The mobile phase of n-hexane: ethyl acetate (93:7) was used for terpenoid detection, meanwhile ethyl acetate: formic acid: water (90:5:5) were used for flavonoid detection. The result was further investigated under visible light and UV light 254 & 366 nm followed by anisaldehyde-H2SO4 spray reagent and stigmasterol as standard for terpenoid detection, and sitroborat spray reagent with quercitrin standard for flavonoid detection. Based on detection by TLC method, ethanolic extract D. pentandra leaves did not contain any alkaloids. D. pentandra contains stigmasterol which is indicated by pink spots after being sprayed with sulfuric acid anisaldehyde (hRf 70). The results of the study also showed that D. pentandra contained quercitrin which was indicated by yellowish green spots after being sprayed with sitroborate (hRf 60) and observed under 366 nm UV lamp.

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Abstract

Mistletoe has been acknowledged as medicinal plant for traditional treatment because it possesses beneficial compound which potentially aid various diseases. Despite its destructive nature, each species of mistletoes can exhibit different beneficial compounds. Exploratory research has been conducted for detection secondary metabolites of Dendrophthoe pentandra that grow on the Bodhi tree (Ficus religiosa L.) at Universitas Gadjah Mada, Yogyakarta, Indonesia. The aim of this research is to detect secondary metabolites which potentially for medicational purpose in D. pentandra using thin layer chromatography method. The mistletoes sample was obtained from the Bodhi tree, authentication of the species of mistletoes was carried out at the Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada. D. pentandra leaf was washed and dried in a dryer cabinet at 50o C, it was subsequently powdered. Amount of 5 g of leaf powder was macerated with 50 ml of 95% ethanol. Analysis of chemical compounds was carried out using the Thin Layer Chromatography (TLC) method with silica gel 60 F254 as a stationary phase and a mobile phase suitable for terpenoids and flavonoids compounds. The mobile phase of n-hexane: ethyl acetate (93:7) was used for terpenoid detection, meanwhile ethyl acetate: formic acid: water (90:5:5) were used for flavonoid detection. The result was further investigated under visible light and UV light 254 & 366 nm followed by anisaldehyde-H2SO4 spray reagent and stigmasterol as standard for terpenoid detection, and sitroborat spray reagent with quercitrin standard for flavonoid detection. Based on detection by TLC method, ethanolic extract D. pentandra leaves did not contain any alkaloids. D. pentandra contains stigmasterol which is indicated by pink spots after being sprayed with sulfuric acid anisaldehyde (hRf 70). The results of the study also showed that D. pentandra contained quercitrin which was indicated by yellowish green spots after being sprayed with sitroborate (hRf 60) and observed under 366 nm UV lamp.

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Item Type:	Article
AGROVOC Term:	terpenoids
AGROVOC Term:	flavonoids
AGROVOC Term:	medicinal plants
AGROVOC Term:	leaves
AGROVOC Term:	Ficus religiosa
AGROVOC Term:	secondary metabolites
AGROVOC Term:	plant extracts
AGROVOC Term:	detection
AGROVOC Term:	chromatography
AGROVOC Term:	extraction
Geographical Term:	Indonesia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	20 May 2026 06:33
Last Modified:	20 May 2026 06:33
URI:	http://webagris.upm.edu.my/id/eprint/3910

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