Evaluation of the expression patterns of mesocarp-specific promoter MSP-C6 in transiently transformed oil palm tissues

Siti Suriawati Badai, . and Omar Abd Rasid, . and Mat Yunus Abdul Masani, . and Noor Azmi Shaharuddin, . and Mohd Puad Abdullah, . and Ghulam Kadir Ahmad Parveez, . and Chai, Ling Ho (2024) Evaluation of the expression patterns of mesocarp-specific promoter MSP-C6 in transiently transformed oil palm tissues. Journal of Tropical Plant Physiology (JTPP) (Malaysia), 16 (2). pp. 45-58. ISSN 1985-0484

Genetic manipulation of oil synthesis in the mesocarp tissue of oil palm may ensure sustainability of palm oil production and fulfil the high demand for palm oil. The suitability of newly isolated promoters in driving a gene must be tested before they can be used for gene manipulation, hence an easy, rapid and manageable method for large sample size is needed. This study aimed to examine the mesocarp-specific expression of the MSP-C6 promoter in oil palm. The MSP-C6 promoter fragments of varying lengths were tested in regulating the red fluorescent protein (DsRED) gene in oil palm tissues through biolistic- and polyethylene glycol (PEG)-mediated transient transformation. Our results showed that the MSP-C6 promoter fragments were not able to express the DsRED gene in the bombarded mesocarp tissue. However, the DsRED signals were observed in the bombarded polyembryoid tissue driven by the MSP-C6-F1 (2014 bp) promoter. Meanwhile, the DsRED expression was also not observed in all the protoplasts isolated from mesocarp tissue transformed by PEG-mediated method. Based on these results, the transient assays of MSP-C6 promoter fragments in oil palm tissues may need further optimisation. This is important to confirm the mesocarp-specific expression of the MSP-C6 promoter as in previous stable transformation of tomato plants. Confirmation of the tissue specificity of the MSP-C6 promoter is crucial before this promoter can be used for the modification of lipid composition in mesocarp tissue oil palm fruit.

Full text available from: Official URL: https://www.jtpp.org.my/volume/16_2/vol16_2_articl...

Abstract

Genetic manipulation of oil synthesis in the mesocarp tissue of oil palm may ensure sustainability of palm oil production and fulfil the high demand for palm oil. The suitability of newly isolated promoters in driving a gene must be tested before they can be used for gene manipulation, hence an easy, rapid and manageable method for large sample size is needed. This study aimed to examine the mesocarp-specific expression of the MSP-C6 promoter in oil palm. The MSP-C6 promoter fragments of varying lengths were tested in regulating the red fluorescent protein (DsRED) gene in oil palm tissues through biolistic- and polyethylene glycol (PEG)-mediated transient transformation. Our results showed that the MSP-C6 promoter fragments were not able to express the DsRED gene in the bombarded mesocarp tissue. However, the DsRED signals were observed in the bombarded polyembryoid tissue driven by the MSP-C6-F1 (2014 bp) promoter. Meanwhile, the DsRED expression was also not observed in all the protoplasts isolated from mesocarp tissue transformed by PEG-mediated method. Based on these results, the transient assays of MSP-C6 promoter fragments in oil palm tissues may need further optimisation. This is important to confirm the mesocarp-specific expression of the MSP-C6 promoter as in previous stable transformation of tomato plants. Confirmation of the tissue specificity of the MSP-C6 promoter is crucial before this promoter can be used for the modification of lipid composition in mesocarp tissue oil palm fruit.

[error in script]

Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	palm oils
AGROVOC Term:	lipids
AGROVOC Term:	mesocarp
AGROVOC Term:	gene expression
AGROVOC Term:	genetic transformation
AGROVOC Term:	bio-assays
AGROVOC Term:	promoters
AGROVOC Term:	biolistics
AGROVOC Term:	protoplasts
Geographical Term:	Malaysia
Uncontrolled Keywords:	Biolistic-mediated transient transformation, mesocarp-specific promoter, oil palm, polyethylene glycol (PEG)-mediated transient transformation
Depositing User:	Ms. Azariah Hashim
Date Deposited:	16 Jun 2026 09:02
Last Modified:	16 Jun 2026 09:02
URI:	http://webagris.upm.edu.my/id/eprint/3912

Actions (login required)

View Item