Production of eurycomalactone and scopoletin in different parts of 2 years old Eurycoma Longifolia (Tongkat Ali) seedlings and tissue culture plantlets

Nor Hasnida Hassan, . and Rosdi Koter, . and Nazirah Abdullah, . and Haliza Ismail, . and Rozidah Khalid, . and Rohani Abdullah, . and Noor Ismawaty Nordin, . and Mohd Rani Awang, . (2023) Production of eurycomalactone and scopoletin in different parts of 2 years old Eurycoma Longifolia (Tongkat Ali) seedlings and tissue culture plantlets. International Journal of Agriculture, Forestry and Plantation (Malaysia), 13. pp. 142-153. ISSN 2462-1757

Eurycoma longifolia (Tongkat Ali) is one of the most widely used herbs in Malaysia and other Asian country and its demand is increasing rapidly. As raw material supplies from natural resources begin to decreasing, it is necessary to cultivate them widely to meet the demands of the herbal and pharmaceutical industries. For this purpose, seedlings are the main requirement because the harvest of E. longifolia involves the whole tree. The production of seedlings through the tissue culture method is one of the commonly used methods to contribute in increasing the supply of planting material. Plantations for forest species and herbal plants are needed to address the problem of insufficient supply of raw materials to meet the demands of the timber, herbal and pharmaceutical industries. Quality of E. longifolia raw material for the herbal and pharmaceutical product muchly depends on the production of chemical compounds in the planting material. In this study, eurycomalactone and scopoletin is our main focus, since this compounds are also valuable compound of E. longifolia and have substantial effect on human health. Stem and leaves also evaluated because normally for E. longifolia products the roots were harvested. If stem and leaves have comparable amount of bioactive compound so we can save E. longifolia trees from destructive method. In this study, different parts which is stem, roots and leaves of 2 years old E. longifolia seedlings and tissue culture plantlets grown in the nursery were evaluated for the production of eurycomalactone and scopoletin using High Performance Liquid Chromatography (HPLC). The results of chemical analysis showed that eurycomalactone and scopoletin were found in root and stem samples except leaf samples from tissue culture plantlets and seedlings.

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Abstract

Eurycoma longifolia (Tongkat Ali) is one of the most widely used herbs in Malaysia and other Asian country and its demand is increasing rapidly. As raw material supplies from natural resources begin to decreasing, it is necessary to cultivate them widely to meet the demands of the herbal and pharmaceutical industries. For this purpose, seedlings are the main requirement because the harvest of E. longifolia involves the whole tree. The production of seedlings through the tissue culture method is one of the commonly used methods to contribute in increasing the supply of planting material. Plantations for forest species and herbal plants are needed to address the problem of insufficient supply of raw materials to meet the demands of the timber, herbal and pharmaceutical industries. Quality of E. longifolia raw material for the herbal and pharmaceutical product muchly depends on the production of chemical compounds in the planting material. In this study, eurycomalactone and scopoletin is our main focus, since this compounds are also valuable compound of E. longifolia and have substantial effect on human health. Stem and leaves also evaluated because normally for E. longifolia products the roots were harvested. If stem and leaves have comparable amount of bioactive compound so we can save E. longifolia trees from destructive method. In this study, different parts which is stem, roots and leaves of 2 years old E. longifolia seedlings and tissue culture plantlets grown in the nursery were evaluated for the production of eurycomalactone and scopoletin using High Performance Liquid Chromatography (HPLC). The results of chemical analysis showed that eurycomalactone and scopoletin were found in root and stem samples except leaf samples from tissue culture plantlets and seedlings.

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Item Type:	Article
AGROVOC Term:	stems
AGROVOC Term:	roots
AGROVOC Term:	leaves
AGROVOC Term:	plant parts
AGROVOC Term:	integrated plant production
AGROVOC Term:	tissue culture
AGROVOC Term:	chemical analysis
AGROVOC Term:	pharmaceutical industry
AGROVOC Term:	seedlings
AGROVOC Term:	bioactive compounds
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	10 Jun 2026 12:38
Last Modified:	10 Jun 2026 12:38
URI:	http://webagris.upm.edu.my/id/eprint/3998

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