Effect of combination Naphthalene Acetic Acid (NAA) and 6-Benzylaminopurine (BAP) on in vitro micropropagation of rice (Oryza sativa l.) cv. MR269

Tarmizi N.H.A., . and Mazlan N.U., . and Dolhaji N.H., . and Latif P.A., . (2023) Effect of combination Naphthalene Acetic Acid (NAA) and 6-Benzylaminopurine (BAP) on in vitro micropropagation of rice (Oryza sativa l.) cv. MR269. International Journal of Agriculture, Forestry and Plantation (Malaysia), 13. pp. 170-179. ISSN 2462-1757

Rice (Oryza sativa L.) is among the most significant food plants globally, with 90% of the total production are from Asia. MR269 variety is high-yielding and resistant to many diseases. However, there are limited study on the in vitro technique for the optimum plant propagation. Tissue culture technique is an efficient approach to improve the rice regeneration by optimization of plant growth regulators. This study is carried out to observe the effect of combination of NAA and BAP hormone and identify the best combination of NAA and BAP hormone for in vitro micropropagation of rice (Oryza sativa L.) cultivar MR269 towards the height of plantlets, number of shoots, number of roots, length of roots, and fresh weight. Five different combinations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 mg/L) and NAA (0.1, 0.2, 0.3, 0.4, 0.5 mg/L) observed after 4 weeks. Significant results obtained between the parameters where maximum number of roots (15.25 ± 1.89), length of roots (1.8 ± 0.25cm) and height of plantlets (13.88 ± 0.47cm) were obtained in MS media supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA. The highest number of shoots (7.5 ± 0.29) and fresh weight (0.21 ± 0.05g) were observed in MS medium supplemented with 3.0 mg/L BAP + 0.3 mg/L NAA and 5.0 mg/L BAP + 0.5 mg/L NAA respectively. The best combination of hormone for the efficient of rice regeneration was in the MS supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA

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Abstract

Rice (Oryza sativa L.) is among the most significant food plants globally, with 90% of the total production are from Asia. MR269 variety is high-yielding and resistant to many diseases. However, there are limited study on the in vitro technique for the optimum plant propagation. Tissue culture technique is an efficient approach to improve the rice regeneration by optimization of plant growth regulators. This study is carried out to observe the effect of combination of NAA and BAP hormone and identify the best combination of NAA and BAP hormone for in vitro micropropagation of rice (Oryza sativa L.) cultivar MR269 towards the height of plantlets, number of shoots, number of roots, length of roots, and fresh weight. Five different combinations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 mg/L) and NAA (0.1, 0.2, 0.3, 0.4, 0.5 mg/L) observed after 4 weeks. Significant results obtained between the parameters where maximum number of roots (15.25 ± 1.89), length of roots (1.8 ± 0.25cm) and height of plantlets (13.88 ± 0.47cm) were obtained in MS media supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA. The highest number of shoots (7.5 ± 0.29) and fresh weight (0.21 ± 0.05g) were observed in MS medium supplemented with 3.0 mg/L BAP + 0.3 mg/L NAA and 5.0 mg/L BAP + 0.5 mg/L NAA respectively. The best combination of hormone for the efficient of rice regeneration was in the MS supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA

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Item Type:	Article
AGROVOC Term:	rice
AGROVOC Term:	Oryza sativa
AGROVOC Term:	micropropagation
AGROVOC Term:	tissue culture
AGROVOC Term:	regeneration
AGROVOC Term:	6-benzylaminopurine
AGROVOC Term:	root length
AGROVOC Term:	plant height
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	11 Jun 2026 03:07
Last Modified:	11 Jun 2026 03:07
URI:	http://webagris.upm.edu.my/id/eprint/4002

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