Simplified QuEChERS technique followed by UHPLC-MS/MS analysis for the determination bisphenol A in whole and powdered milk

Souza, P. S. and Krauss, T. M. and Sartori, A. V. and Abrantes, S. M. P. (2023) Simplified QuEChERS technique followed by UHPLC-MS/MS analysis for the determination bisphenol A in whole and powdered milk. International Food Research Journal (Malaysia), 30. pp. 524-535. ISSN 2231 7546

Bisphenol A (BPA), an important endocrine disruptor, is employed in the manufacturing of many materials such as food packaging. The ingestion of contaminated food is considered the most relevant form of exposure to this compound. Data concerning the presence of this contaminant in milk in Brazil, however, are still lacking. In this context, an analytical method for the determination of BPA in ultrahigh temperature (UHT) milk, pasteurised milk, and milk powder was developed. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method was used for sample treatment, and BPA was determined by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The method was selective for BPA in all investigated milk samples. No matrix effects were observed, thus indicating that calibration curves could be prepared in solvent for routine analysis. The method presented satisfactory accuracy, with recovery values between 78 and 94%. Relative standard deviation values obtained in the repeatability (11%) and intermediate precision (4.8%) studies were also satisfactory (HorRatr and HorRatR values less than 2). The analyte limits of detection and quantification were 0.12 and 0.36 ng/g for while milk, and 0.40 and 1.20 ng/g for reconstituted milk powder (1:10), respectively, and the sensitivity of the analytical method was considered adequate for the purpose of the present work.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(02)%202023/21%20-...

Abstract

Bisphenol A (BPA), an important endocrine disruptor, is employed in the manufacturing of many materials such as food packaging. The ingestion of contaminated food is considered the most relevant form of exposure to this compound. Data concerning the presence of this contaminant in milk in Brazil, however, are still lacking. In this context, an analytical method for the determination of BPA in ultrahigh temperature (UHT) milk, pasteurised milk, and milk powder was developed. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method was used for sample treatment, and BPA was determined by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The method was selective for BPA in all investigated milk samples. No matrix effects were observed, thus indicating that calibration curves could be prepared in solvent for routine analysis. The method presented satisfactory accuracy, with recovery values between 78 and 94%. Relative standard deviation values obtained in the repeatability (11%) and intermediate precision (4.8%) studies were also satisfactory (HorRatr and HorRatR values less than 2). The analyte limits of detection and quantification were 0.12 and 0.36 ng/g for while milk, and 0.40 and 1.20 ng/g for reconstituted milk powder (1:10), respectively, and the sensitivity of the analytical method was considered adequate for the purpose of the present work.

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Item Type:	Article
AGROVOC Term:	whole milk
AGROVOC Term:	dried milk
AGROVOC Term:	milk
AGROVOC Term:	sampling
AGROVOC Term:	statistical methods
AGROVOC Term:	food analysis
AGROVOC Term:	laboratories
AGROVOC Term:	food safety
Geographical Term:	Brazil
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	08 Jul 2025 02:10
Last Modified:	08 Jul 2025 02:10
URI:	http://webagris.upm.edu.my/id/eprint/574

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