Citation
E.F.A., Susanti and E., Susilowati and Tri Agus, Siswoyo. (2022) Effect of germination period on the antioxidant activities and angiotensin-I converting enzyme inhibitory of Indonesian black rice. Food Research (Malaysia), 6 (4). pp. 59-67. ISSN 2550-2166
Abstract
Indonesian black rice (Oryza sativa L., Ketan Hitam-2) is pigmented rice with high potency as a nutraceutical compound, especially with its high protein content. The effect of the germination period on antioxidant activities and angiotensin I-converting enzyme (ACE-I) inhibitory of Indonesian black rice seed protein was studied to determine its potential use as a nutraceutical ingredient. In this study, the bioactive peptide was produced by protein modification through the germination process for 0, 2, 4, and 6 days. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition were conducted to determine the changes in protein during the germination period. Two antioxidant methods (ABTS•+ and OH• ) were performed to determine the antioxidant activity and the defence against radical-mediated DNA damage by hydroxyl. Meanwhile, the antihypertensive potency was analysed by ACE-I inhibitor activity. The results showed that the antioxidant-protected hydroxyl radical-induced oxidative DNA damage and ACE-I inhibitor activities were increased during the germination period. The IC₅₀ value of ABTS•+ and hydroxyl radical scavenging on the sixth-day germination were 28.18 μg/mL, and 24.84 μg/mL, respectively, lower than the control (before germinated). Moreover, the IC₅₀ value of ACE-I Inhibitory activities during the six-day germination was (9.07 μg/mL). The above results indicated that the germination period could increase the activity of bioactive peptides in Indonesian black rice. It might be used in future nutraceuticals and human health applications.
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Abstract
Indonesian black rice (Oryza sativa L., Ketan Hitam-2) is pigmented rice with high potency as a nutraceutical compound, especially with its high protein content. The effect of the germination period on antioxidant activities and angiotensin I-converting enzyme (ACE-I) inhibitory of Indonesian black rice seed protein was studied to determine its potential use as a nutraceutical ingredient. In this study, the bioactive peptide was produced by protein modification through the germination process for 0, 2, 4, and 6 days. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition were conducted to determine the changes in protein during the germination period. Two antioxidant methods (ABTS•+ and OH• ) were performed to determine the antioxidant activity and the defence against radical-mediated DNA damage by hydroxyl. Meanwhile, the antihypertensive potency was analysed by ACE-I inhibitor activity. The results showed that the antioxidant-protected hydroxyl radical-induced oxidative DNA damage and ACE-I inhibitor activities were increased during the germination period. The IC₅₀ value of ABTS•+ and hydroxyl radical scavenging on the sixth-day germination were 28.18 μg/mL, and 24.84 μg/mL, respectively, lower than the control (before germinated). Moreover, the IC₅₀ value of ACE-I Inhibitory activities during the six-day germination was (9.07 μg/mL). The above results indicated that the germination period could increase the activity of bioactive peptides in Indonesian black rice. It might be used in future nutraceuticals and human health applications.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oryza sativa |
| AGROVOC Term: | natural antioxidants > natural antioxidants Prefer using antioxidantsantioxidants |
| AGROVOC Term: | germination |
| AGROVOC Term: | sampling |
| AGROVOC Term: | gel electrophoresis |
| AGROVOC Term: | statistical analysis > statistical analysis Prefer using statistical methodsstatistical methods |
| AGROVOC Term: | researchers > researchers Prefer using scientistsscientists |
| AGROVOC Term: | farmers |
| AGROVOC Term: | nutritional value > nutritional value Prefer using nutritive valuenutritive value |
| Geographical Term: | Indonesia |
| Depositing User: | Ms. Azariah Hashim |
| Date Deposited: | 10 Oct 2024 08:52 |
| Last Modified: | 10 Oct 2024 08:52 |
| URI: | http://webagris.upm.edu.my/id/eprint/647 |
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