Citation
Wang, Daniel I. C. and Gbewonyo, Kodzo. (1982) Modern concepts of biotechnology/enhanced oxygen transfer in mycelial fermentations. [Proceedings Paper]
Abstract
In an effort to alter the filamentous morphology of Penicillium chrysogenum cells, a technique was developed to confine the growth of the mycelia to spherical beads. Celite was found to provide an effective support for the growth of the cells. The internal structure of celite was shown to possess an interconnected network of pores which are accessible for entrapping mycelial cells and spores. A simple procedure was developed to inoculate the beads by entrapment of spores. The cells of Penicillium chrysogenum were grown confined to these beads in shake flask cultures and bubble column fermentor. Significant increases in the mycelial growth were obtained with final cell concentrations up to 60 g/l in shake flasks. These concentrations nearly doubled the final cell densities attainable in the conventional freely suspended cultures grown in the absence of beads. The mycelial loadings attained amounted to 0.55 gram of cells per gram of bead. In a bubble column fermentor, high cell concentrations were obtained when the cells were confined to the celite beads. Oxygen transfer coefficients measured at equal aeration rates were three times greater at all cell concentrations for the confined growth cells on the bead as compared to free cell cultures.
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Abstract
In an effort to alter the filamentous morphology of Penicillium chrysogenum cells, a technique was developed to confine the growth of the mycelia to spherical beads. Celite was found to provide an effective support for the growth of the cells. The internal structure of celite was shown to possess an interconnected network of pores which are accessible for entrapping mycelial cells and spores. A simple procedure was developed to inoculate the beads by entrapment of spores. The cells of Penicillium chrysogenum were grown confined to these beads in shake flask cultures and bubble column fermentor. Significant increases in the mycelial growth were obtained with final cell concentrations up to 60 g/l in shake flasks. These concentrations nearly doubled the final cell densities attainable in the conventional freely suspended cultures grown in the absence of beads. The mycelial loadings attained amounted to 0.55 gram of cells per gram of bead. In a bubble column fermentor, high cell concentrations were obtained when the cells were confined to the celite beads. Oxygen transfer coefficients measured at equal aeration rates were three times greater at all cell concentrations for the confined growth cells on the bead as compared to free cell cultures.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia TP501 A816 1982 Call number. |
| AGROVOC Term: | biotechnology |
| AGROVOC Term: | fermentation |
| AGROVOC Term: | experimentation |
| AGROVOC Term: | sampling |
| AGROVOC Term: | sterilization |
| AGROVOC Term: | inoculation |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | microorganisms |
| AGROVOC Term: | yield increases |
| AGROVOC Term: | biomass production |
| Depositing User: | Ms. Azariah Hashim |
| Date Deposited: | 15 Oct 2024 04:16 |
| Last Modified: | 15 Oct 2024 04:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/760 |
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