Citation
K. I. Kandasamy, . and Siti Suhaila A. R., . and Rosilah A. A., . (2006) Micropropagation of Shorea leprosula (meranti tembaga) using a temporary immersion system (RITA system). In: 8th Round-Table Conference on Dipterocarps: Dipterocarps - Enhancing Capacities in Sustainable Development and Poverty Alleviation, 15-17 Nov 2005, Ho Chi Minh City (Vietnam).
Abstract
Shorea leprosula belongs to the family Dipterocarpaceae and its synonyms are Hopea maranti Miq. Shorea maranti Burck Shorea astrostricta Scort. Ex. Foxw and the common name (in Malaysia) is meranti tembaga. This dipterocarp species has been propagated mostly through seeds and cuttings; however the seed production is erratic and infrequent. Furthermore the seeds deteriorate rapidly in storage (a behavior associated with recalcitrant seeds). In addition production of cuttings from mature trees is difficult and plagiotropic growth of shoots from cuttings is common. In recent years many researchers have been reporting on the use of tissue culture techniques to address these problems however most used embryo or axillary buds as starting material. Nevertheless there is an increasing interest in clonal propagation of dipterocarp species by tissue culture because the forest of Dipterocarpaceae has been disappearing rapidly. Thus the development of an efficient culture method for mass production of dipterocarp is important. Although it was reported that this species does not tolerate watery soil but it is worth noting that the water uptake by this plant is quite high. Thus we believe that this plant could be manipulated (for propagation via micropropagation) using a suitable temporary-immersion system. In this paper we will be reporting the ability to successfully micropropagate Shorea leprosula by using a temporary-immersion technique the RITA system. Shoot cultures from existing tissue culture material (maintained in semi-solid medium) at FRIM’s Tissue Culture Laboratory were used as starting material for this study. Subsequent shoots obtained from this study were successfully rooted and acclimatised using our proprietary weaning chamber in our greenhouse.
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Abstract
Shorea leprosula belongs to the family Dipterocarpaceae and its synonyms are Hopea maranti Miq. Shorea maranti Burck Shorea astrostricta Scort. Ex. Foxw and the common name (in Malaysia) is meranti tembaga. This dipterocarp species has been propagated mostly through seeds and cuttings; however the seed production is erratic and infrequent. Furthermore the seeds deteriorate rapidly in storage (a behavior associated with recalcitrant seeds). In addition production of cuttings from mature trees is difficult and plagiotropic growth of shoots from cuttings is common. In recent years many researchers have been reporting on the use of tissue culture techniques to address these problems however most used embryo or axillary buds as starting material. Nevertheless there is an increasing interest in clonal propagation of dipterocarp species by tissue culture because the forest of Dipterocarpaceae has been disappearing rapidly. Thus the development of an efficient culture method for mass production of dipterocarp is important. Although it was reported that this species does not tolerate watery soil but it is worth noting that the water uptake by this plant is quite high. Thus we believe that this plant could be manipulated (for propagation via micropropagation) using a suitable temporary-immersion system. In this paper we will be reporting the ability to successfully micropropagate Shorea leprosula by using a temporary-immersion technique the RITA system. Shoot cultures from existing tissue culture material (maintained in semi-solid medium) at FRIM’s Tissue Culture Laboratory were used as starting material for this study. Subsequent shoots obtained from this study were successfully rooted and acclimatised using our proprietary weaning chamber in our greenhouse.
Additional Metadata
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 495 D564R859 2005 (095003273) Call Number |
| AGROVOC Term: | Shorea |
| AGROVOC Term: | Dipterocarpaceae |
| AGROVOC Term: | Dipterocarps |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Shoot tip culture |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | In vitro plantlets |
| Geographical Term: | MALAYSIA |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:52 |
| URI: | http://webagris.upm.edu.my/id/eprint/7693 |
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