Isolation and characterisation of collagen from fringescale sardinella (Sardinella fimbriata) waste materials


Citation

F. S. Hamdan, . and N. M. Sarbon, . Isolation and characterisation of collagen from fringescale sardinella (Sardinella fimbriata) waste materials. pp. 133-140. ISSN 2231-7546

Abstract

The present work was aimed to isolate and characterise collagen from fringescale sardinella (Sardinella fimbriata) waste materials using two extraction methods; acid soluble collagen (ASC) and pepsin soluble collagen (PSC). The physical and chemical properties of extracted collagens were characterised in terms of protein concentration structural solubility and morphological properties. The yields of extracted collagen were 7.48 4.84 for ASC and 0.94 0.22 for PSC. The protein concentrations for ASC and PSC were significantly (p 0.05) lower than commercial collagen (from tilapia scale). Functional groups for extracted collagens such as amide A (3400-3440 cm-) amide II (1400-1600 cm-) and amide III (1200-1500 cm ) bands were in the same range as commercial collagen. A higher solubility of commercial collagen and ASC was observed under acidic conditions while PSC was highly soluble under alkaline conditions. Morphological study showed that the collagen had a flaky and fibrillary structure. ASC and PSC exhibited similar properties to the commercial collagen albeit being of lower values. Therefore the collagen extracted from fringescale sardinella demonstrated potential for use as an alternative collagen from marine sources.


Download File

Full text available from:

Abstract

The present work was aimed to isolate and characterise collagen from fringescale sardinella (Sardinella fimbriata) waste materials using two extraction methods; acid soluble collagen (ASC) and pepsin soluble collagen (PSC). The physical and chemical properties of extracted collagens were characterised in terms of protein concentration structural solubility and morphological properties. The yields of extracted collagen were 7.48 4.84 for ASC and 0.94 0.22 for PSC. The protein concentrations for ASC and PSC were significantly (p 0.05) lower than commercial collagen (from tilapia scale). Functional groups for extracted collagens such as amide A (3400-3440 cm-) amide II (1400-1600 cm-) and amide III (1200-1500 cm ) bands were in the same range as commercial collagen. A higher solubility of commercial collagen and ASC was observed under acidic conditions while PSC was highly soluble under alkaline conditions. Morphological study showed that the collagen had a flaky and fibrillary structure. ASC and PSC exhibited similar properties to the commercial collagen albeit being of lower values. Therefore the collagen extracted from fringescale sardinella demonstrated potential for use as an alternative collagen from marine sources.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Collagen
AGROVOC Term: Isolation
AGROVOC Term: Extraction in chemical analysis
AGROVOC Term: Sardinella fimbriata
AGROVOC Term: Wastes
AGROVOC Term: Physical properties
AGROVOC Term: Chemical properties
AGROVOC Term: Fish protein concentrate
AGROVOC Term: Solubility
AGROVOC Term: Microstructure (morphology)
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:53
URI: http://webagris.upm.edu.my/id/eprint/7774

Actions (login required)

View Item View Item
Agris UPM is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.