Isolation and characterization of serum immunoglobulins from kalbasu (Labeo calbasu) (Hamilton 1822)


Citation

Behera S. K., . and Mohanty J., . and Swain T., . and Garnayak S. K., . Isolation and characterization of serum immunoglobulins from kalbasu (Labeo calbasu) (Hamilton 1822). pp. 481-489. ISSN 0116-6514

Abstract

The Ig (Ig) from the serum of kalbasu (Labeo calbasu Cyprinidae) was isolated and characterized during the present investigation. Kalbasu of 500-800 g size were immunized with Bovine Berum Albumin (BSA) to prepare immune sera. The Ig was isolated from the immune sera by an immunoaffinity column of BSA-Sepharose 4B. The purity and homogeneity of the isolated sample were confirmed by observing a single band in native gradient polyacrylamide gel electrophoresis. This also indicated the presence of only single type of Ig in kalbasu. The molecular weight of native Ig molecule was determined from the gel to be 857 kDa. In Sodium dodecyl sulphate polyacrylamide gel electrophoresis (E) the constituent heavy (H) and light (L) polypeptide chains of the Ig molecules were identified. There were only one type of H chain with a molecular weight of 78 kDa and two types of L chain with molecular weights of 27 kDa and 26 kDa. The antiserum against the kalbasu Ig was raised in a rabbit and adsorbed with 10 kalbasu liver tissue homogenate in order to enhance its specificity. By an indirect ELISA standardized using this adsorbed rabbit antiserum the normal serum Ig concentration in kalbasu was estimated to be 2.82 mg.ml-1 (n22) which is around 8 of the total serum proteins.


Download File

Full text available from:

Abstract

The Ig (Ig) from the serum of kalbasu (Labeo calbasu Cyprinidae) was isolated and characterized during the present investigation. Kalbasu of 500-800 g size were immunized with Bovine Berum Albumin (BSA) to prepare immune sera. The Ig was isolated from the immune sera by an immunoaffinity column of BSA-Sepharose 4B. The purity and homogeneity of the isolated sample were confirmed by observing a single band in native gradient polyacrylamide gel electrophoresis. This also indicated the presence of only single type of Ig in kalbasu. The molecular weight of native Ig molecule was determined from the gel to be 857 kDa. In Sodium dodecyl sulphate polyacrylamide gel electrophoresis (E) the constituent heavy (H) and light (L) polypeptide chains of the Ig molecules were identified. There were only one type of H chain with a molecular weight of 78 kDa and two types of L chain with molecular weights of 27 kDa and 26 kDa. The antiserum against the kalbasu Ig was raised in a rabbit and adsorbed with 10 kalbasu liver tissue homogenate in order to enhance its specificity. By an indirect ELISA standardized using this adsorbed rabbit antiserum the normal serum Ig concentration in kalbasu was estimated to be 2.82 mg.ml-1 (n22) which is around 8 of the total serum proteins.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Labeo
AGROVOC Term: Labeo calbasu
AGROVOC Term: Aquatic animals
AGROVOC Term: Rabbits
AGROVOC Term: Albumins
AGROVOC Term: Immunoglobulins
AGROVOC Term: Isolation
AGROVOC Term: Western blotting
AGROVOC Term: Blood proteins
AGROVOC Term: Blood serum
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:53
URI: http://webagris.upm.edu.my/id/eprint/7794

Actions (login required)

View Item View Item
Agris UPM is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.