Effect of low temperature on metabolic enzymes and HSP-70 expression of coldwater fish Barilius bendelisis

Kapila Rajeev, . and Pant Rochna, . and Gaur A. K., . and Mahanta P. C., . Effect of low temperature on metabolic enzymes and HSP-70 expression of coldwater fish Barilius bendelisis. pp. 125-136. ISSN 0116-6514

The levels of key metabolic enzymes viz. Glucose 6 phosphate dehydrogenase Lactate dehydrogenase Pyruvate kinase Acetylcholine esterase and ATPase were monitored at varying time intervals in coldwater fish Barilius bendelisis exposed to 5C. The levels of these enzymes increased to 236.23 28.22 68.66 83.59 and 143.55 respectively as compared to control group of fishes during 96hrs of rearing in cold. The enzymatic values of pyruvate kinase lactate dehydrogenase and ATPase levels increased considerably (P0.01) just after 24 hrs of treatment while glucose 6 phosphate dehydrogenase and esterase increased significantly (P0.01) after 48 hrs of cold treatment. Isozyme profiling of above enzymes also supports the data visually. Moreover two more isoforms of Pyruvate kinase were also observed in cold adapted fish specimens than in control group. Twelve protein bands were also visualized when the samples from test fishes were run on the SDS-PAGE one band of 29kD at Rf of 0.63 exhibited relatively high intensity in the fish reared at cold temperature as compared to control group. Similarly elevated levels of Hsp-70 were also detected (p0.05) due to the cold stress given to the fishes for a period of 24- 96 hrs that also served as an early indicator of temperature stress in fish and played an important role in cold adaptation.

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Abstract

The levels of key metabolic enzymes viz. Glucose 6 phosphate dehydrogenase Lactate dehydrogenase Pyruvate kinase Acetylcholine esterase and ATPase were monitored at varying time intervals in coldwater fish Barilius bendelisis exposed to 5C. The levels of these enzymes increased to 236.23 28.22 68.66 83.59 and 143.55 respectively as compared to control group of fishes during 96hrs of rearing in cold. The enzymatic values of pyruvate kinase lactate dehydrogenase and ATPase levels increased considerably (P0.01) just after 24 hrs of treatment while glucose 6 phosphate dehydrogenase and esterase increased significantly (P0.01) after 48 hrs of cold treatment. Isozyme profiling of above enzymes also supports the data visually. Moreover two more isoforms of Pyruvate kinase were also observed in cold adapted fish specimens than in control group. Twelve protein bands were also visualized when the samples from test fishes were run on the SDS-PAGE one band of 29kD at Rf of 0.63 exhibited relatively high intensity in the fish reared at cold temperature as compared to control group. Similarly elevated levels of Hsp-70 were also detected (p0.05) due to the cold stress given to the fishes for a period of 24- 96 hrs that also served as an early indicator of temperature stress in fish and played an important role in cold adaptation.

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Item Type:	Article
AGROVOC Term:	Barilius bendelisis
AGROVOC Term:	Cyprinidae
AGROVOC Term:	Fishes
AGROVOC Term:	Enzymatic analysis
AGROVOC Term:	Enzymes
AGROVOC Term:	Water temperature
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Pyruvate kinase
AGROVOC Term:	Lactate dehydrogenase
AGROVOC Term:	ATPase
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/7947

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