Citation
Phu Truong Quoc, . and Son Nguyen Hung, . and Oanh Dang Thi Hoang, . and Bondad-Reantaso Melba G., . Field study on transmission of acute hepatopancreatic necrosis syndrome (AHPNS) in infected ponds. pp. 293-301. ISSN 0116-6514
Abstract
A field transmission experiment for acute hepatopancreatic necrosis syndrome (AHPNS) in whiteleg shrimp (Penaeus vannamei) was set up in two AHPNS-infected ponds. Six hapas were placed in each pond; of these three were stocked with healthy shrimp (to investigate transmission via water) and the other three were stocked with both healthy and AHPNS-infected shrimp (to investigate transmission via water and cohabitation). At 10 days post stocking healthy shrimp in both treatments showed typical signs of AHPNS pathology as seen in naturally infected shrimp in ponds. Histopathological analysis revealed rounding and sloughing of hepatopancreatic (HP) epithelial cells reduction in epithelium height loss of certain cell types (B- F- and R-cells) and severe haemocytic infiltration around HP tubules. Mortalities were noted in all experimental hapas; however mortality rates in hapas stocked with both healthy and AHPNS-infected shrimp were higher than in hapas stocked with healthy shrimp only. Isolates of Vibrio bacteria obtained from infected shrimp were identified as V. parahaemolyticus. Polymerase chain reaction (PCR) analysis detected the thl gene from isolates of V. parahaemolyticus but not the thd or trh genes.
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Abstract
A field transmission experiment for acute hepatopancreatic necrosis syndrome (AHPNS) in whiteleg shrimp (Penaeus vannamei) was set up in two AHPNS-infected ponds. Six hapas were placed in each pond; of these three were stocked with healthy shrimp (to investigate transmission via water) and the other three were stocked with both healthy and AHPNS-infected shrimp (to investigate transmission via water and cohabitation). At 10 days post stocking healthy shrimp in both treatments showed typical signs of AHPNS pathology as seen in naturally infected shrimp in ponds. Histopathological analysis revealed rounding and sloughing of hepatopancreatic (HP) epithelial cells reduction in epithelium height loss of certain cell types (B- F- and R-cells) and severe haemocytic infiltration around HP tubules. Mortalities were noted in all experimental hapas; however mortality rates in hapas stocked with both healthy and AHPNS-infected shrimp were higher than in hapas stocked with healthy shrimp only. Isolates of Vibrio bacteria obtained from infected shrimp were identified as V. parahaemolyticus. Polymerase chain reaction (PCR) analysis detected the thl gene from isolates of V. parahaemolyticus but not the thd or trh genes.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Prawns and shrimps |
| AGROVOC Term: | Penaeus |
| AGROVOC Term: | Penaeus vannamei |
| AGROVOC Term: | Aquaculture |
| AGROVOC Term: | Shellfish culture |
| AGROVOC Term: | Bacterial diseases |
| AGROVOC Term: | Disease transmission |
| AGROVOC Term: | Field experimentation |
| AGROVOC Term: | Ponds |
| AGROVOC Term: | Histopathology |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/8005 |
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