Purification of recombinant peroxidase from Thermobifida fusca IP1 for -carotene degradation into industrial flavouring agents

Naz I., . and Raza M. A., . and Ahmed S., . and Perveen I., . and Sehar S., . Purification of recombinant peroxidase from Thermobifida fusca IP1 for -carotene degradation into industrial flavouring agents. pp. 731-736. ISSN 2231-7546

Carotenoids are important colored components of food with several essential health benefits. Because of their characteristic fruity aroma the cleavage products of carotenoids are being used as flavouring agents in the food industry. Enzymes are needed to get cleavage products of carotenoids in order to avoid the very laborious and costly extraction processes of particular aroma compounds such as - and -ionone directly from their plant sources. So for an easy and cost effective access to flavouring agents the role of enzymes is undeniably crucial. The present work was therefore undertaken to isolate an industrially important recombinant peroxidase enzyme to obtain the flavouring agents. Thermobifida fusca (accession number KM677184) isolated from manure piles was found to effectively degrade -carotene. The gene encoding T. fusca extracellular heme-containing peroxidase (TfuDyP) was amplified from genomic DNA of T. fusca and was cloned in vector pET24b. TfuDyP was expressed heterologously in E. coli (BL21) and a recombinant enzyme was used for the transformation of -carotene into volatile products which were identified by the GC-MS (Gas Chromatography-Mass Spectrophotometry). The enzyme was found effective as various volatile degradation products of -carotene such as -ionone -cyclocitral -ionone-5 6-epoxide and dihydroactinidiolide were identified. With these results we are inclined to conclude that the extracellular peroxidase enzyme obtained from T. fusca IP1 has the potential to be used in the food industry. In spite of the various reports on the subject the use of this particular bacterial strain in the enzyme production is being reported for the first time in the present work.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(02)%202019/(40).p...

Abstract

Carotenoids are important colored components of food with several essential health benefits. Because of their characteristic fruity aroma the cleavage products of carotenoids are being used as flavouring agents in the food industry. Enzymes are needed to get cleavage products of carotenoids in order to avoid the very laborious and costly extraction processes of particular aroma compounds such as - and -ionone directly from their plant sources. So for an easy and cost effective access to flavouring agents the role of enzymes is undeniably crucial. The present work was therefore undertaken to isolate an industrially important recombinant peroxidase enzyme to obtain the flavouring agents. Thermobifida fusca (accession number KM677184) isolated from manure piles was found to effectively degrade -carotene. The gene encoding T. fusca extracellular heme-containing peroxidase (TfuDyP) was amplified from genomic DNA of T. fusca and was cloned in vector pET24b. TfuDyP was expressed heterologously in E. coli (BL21) and a recombinant enzyme was used for the transformation of -carotene into volatile products which were identified by the GC-MS (Gas Chromatography-Mass Spectrophotometry). The enzyme was found effective as various volatile degradation products of -carotene such as -ionone -cyclocitral -ionone-5 6-epoxide and dihydroactinidiolide were identified. With these results we are inclined to conclude that the extracellular peroxidase enzyme obtained from T. fusca IP1 has the potential to be used in the food industry. In spite of the various reports on the subject the use of this particular bacterial strain in the enzyme production is being reported for the first time in the present work.

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Item Type:	Article
AGROVOC Term:	Flavourings
AGROVOC Term:	Carotenoids
AGROVOC Term:	Carotenes
AGROVOC Term:	Biodegradation
AGROVOC Term:	Bacteria
AGROVOC Term:	Purification
AGROVOC Term:	Peroxidases
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Volatile compounds
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8099

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