Detection of potentially zoonotic cryptosporidium and giardia among livestock in Sariaya Quezon Philippines

Afable Arianne Lleva, . and De Belen Coquilla Kim Joshua, . and Battad II Zosimo Galang, . and Pornobi Kathleen Ordoñez, . Detection of potentially zoonotic cryptosporidium and giardia among livestock in Sariaya Quezon Philippines. pp. 557-568. ISSN 1511-3701

Livestock plays a great role in the economic development of smallholder farmers. However the population of livestock has been affected by diseases such as cryptosporidiosis and giardiasis. Sariaya has a large production of livestock that is being distributed in Quezon Province. Thus the current study aimed to identify the presence of Cryptosporidium sp. and Giardia sp. in livestock in selected farms of Sariaya Quezon. Risk factors were also assessed in the present study. A total of 103 collected faecal samples from livestock were subjected to microscopic and molecular detection. Faecal samples were processed through Formalin-Ether Concentration Technique (FECT) and Polymerase Chain Reaction. Microscopy results revealed that 14 out of 103 (13.59) samples were positive for Giardia with meant intensity of 13 cysts per gram (cpg) of faeces while molecular detection confirmed that 13 out of 103 (12.62) had amplified for the target gene of Giardia tpi with expected band size of 530 bp. Sequenced samples of G. intestinalis were characterised as assemblages A B and E. Moreover pigs showed the highest prevalence (15.91) while cattle had the highest mean intensity (14 cpg) for Giardia. Cryptosporidium were absent in all the samples. Statistical analysis of the risk factors such as diet feeding floor habit presence of illness and faecal consistency of the animals revealed no significant association (p0.05) in the presence of Giardia. This study revealed the presence of zoonotic Giardia species in the area; however future investigation in other possible risk factors such as the season and age is still recommended.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

Livestock plays a great role in the economic development of smallholder farmers. However the population of livestock has been affected by diseases such as cryptosporidiosis and giardiasis. Sariaya has a large production of livestock that is being distributed in Quezon Province. Thus the current study aimed to identify the presence of Cryptosporidium sp. and Giardia sp. in livestock in selected farms of Sariaya Quezon. Risk factors were also assessed in the present study. A total of 103 collected faecal samples from livestock were subjected to microscopic and molecular detection. Faecal samples were processed through Formalin-Ether Concentration Technique (FECT) and Polymerase Chain Reaction. Microscopy results revealed that 14 out of 103 (13.59) samples were positive for Giardia with meant intensity of 13 cysts per gram (cpg) of faeces while molecular detection confirmed that 13 out of 103 (12.62) had amplified for the target gene of Giardia tpi with expected band size of 530 bp. Sequenced samples of G. intestinalis were characterised as assemblages A B and E. Moreover pigs showed the highest prevalence (15.91) while cattle had the highest mean intensity (14 cpg) for Giardia. Cryptosporidium were absent in all the samples. Statistical analysis of the risk factors such as diet feeding floor habit presence of illness and faecal consistency of the animals revealed no significant association (p0.05) in the presence of Giardia. This study revealed the presence of zoonotic Giardia species in the area; however future investigation in other possible risk factors such as the season and age is still recommended.

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Item Type:	Article
AGROVOC Term:	Livestock
AGROVOC Term:	Bird diseases
AGROVOC Term:	Disease incidence
AGROVOC Term:	Identification
AGROVOC Term:	Microscopy
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Faeces
AGROVOC Term:	Zoonoses
AGROVOC Term:	Cryptosporidium
AGROVOC Term:	Giardia
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8151

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