Enhancing eksotika papaya resistance to dieback disease through quorum quenching

Wee Chien-Yeong, . and Nora'ini Abdullah, . and Johari Sarip, . and Noriha Mat Amin, . and Rogayah Sekeli, . and Nazrul Hisham Nazaruddin, . and Roslinda Abdul Razak, . and Nurain Izzati Saidi, . and Amin Asyraf Tamizi, . and Zaifulfarizal Zulkifli, . Enhancing eksotika papaya resistance to dieback disease through quorum quenching. pp. 1-9. ISSN 1985-0484.

The main objective of this study was to develop transgenic Malaysian Eksotika papaya with enhanced resistance to papaya dieback disease (PDD). Papaya dieback disease which is caused by a Gram-negative bacterium Erwinia mallotivora has adversely affected Malaysia papaya industry and resulted in major production declines and economical losses. To rejuvenate the papaya industry as well as to improve papaya export effective disease prevention strategies are imperative to curb the spread of the pathogen. Apart from the conventional breeding method an alternative strategy by using genetic engineering approach was proposed to produce papaya plants that can silence quorum sensing system of the bacterial pathogen. Hence by disrupting the communication mechanism or known as quorum quenching the disease development was hypothesised to be delayed or inhibited. An acyl-homoserine lactone (AHL) lactonase gene isolated from rhizosphere soil bacteria strain CHB37 which has high quorum quenching potential against E. mallotivora has been isolated validated and transformed into embryogenic papaya calli. Out of 150 putative transgenic lines recovered 70 lines were found positive with the presence of inserted transgenes. Screening of 3-month-old transgenic plants with E. mallotivora confirmed that AHL lactonase CHB37 gene has successfully delayed E. mallotivora infection in 12 transgenic papaya lines. The transgenic plants were able to survive and produce new shoot whereas control plants were mostly dead by the tenth-day post inoculation. It offers a promising outcome nevertheless more screening will be conducted for further validation.

Full text available from: Official URL: https://jtpp.org.my/volume/11_1/vol11_1_article1.p...

Abstract

The main objective of this study was to develop transgenic Malaysian Eksotika papaya with enhanced resistance to papaya dieback disease (PDD). Papaya dieback disease which is caused by a Gram-negative bacterium Erwinia mallotivora has adversely affected Malaysia papaya industry and resulted in major production declines and economical losses. To rejuvenate the papaya industry as well as to improve papaya export effective disease prevention strategies are imperative to curb the spread of the pathogen. Apart from the conventional breeding method an alternative strategy by using genetic engineering approach was proposed to produce papaya plants that can silence quorum sensing system of the bacterial pathogen. Hence by disrupting the communication mechanism or known as quorum quenching the disease development was hypothesised to be delayed or inhibited. An acyl-homoserine lactone (AHL) lactonase gene isolated from rhizosphere soil bacteria strain CHB37 which has high quorum quenching potential against E. mallotivora has been isolated validated and transformed into embryogenic papaya calli. Out of 150 putative transgenic lines recovered 70 lines were found positive with the presence of inserted transgenes. Screening of 3-month-old transgenic plants with E. mallotivora confirmed that AHL lactonase CHB37 gene has successfully delayed E. mallotivora infection in 12 transgenic papaya lines. The transgenic plants were able to survive and produce new shoot whereas control plants were mostly dead by the tenth-day post inoculation. It offers a promising outcome nevertheless more screening will be conducted for further validation.

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Item Type:	Article
AGROVOC Term:	Papayas
AGROVOC Term:	Carica papaya
AGROVOC Term:	Disease prevention
AGROVOC Term:	Dieback
AGROVOC Term:	Plant diseases
AGROVOC Term:	Gram negative bacteria
AGROVOC Term:	Erwinia
AGROVOC Term:	Genetic engineering
AGROVOC Term:	Gene expression
AGROVOC Term:	Disease resistance
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8194

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