Leaf stomatal density and distribution in black pepper under field conditions

Muyang Kevin Tawie Sulok, . and Osumanu Haruna Ahmed, . and Chen Yi Shang, . and Mercer Zehnder Jarroop Augustine, . Leaf stomatal density and distribution in black pepper under field conditions. pp. 24-31. ISSN 1985-0484

Currently there is little information on whether black pepper leaf has more stomata on the underside above side or the stomata are equally distributed. Thus this paper provides vital information on black pepper leaf stomata distribution and density to shed more light on the subject. The objective of this study was to investigate the distribution pattern of stomata on developing and matured leaf of Piper nigrum var. Kuching Semongok Emas and Semongok Aman. To analyse the leaves we used nail polish staining tools combined with a compound microscope incorporated with imaging software. Our analysis indicated that stomata occur only on the lower side of the leaf (hypostomatic). The result on distribution showed that except for matured leaf of the Kuching variety abaxial stomata were found abundantly at the base of the leaf in all varieties and developmental stages. Stomatal densities according to varieties in P. nigrum leaves recorded that the Semongok Aman has the highest stomata count followed by Semongok Emas and Kuching. Differences in stomatal density between leaves of varying developmental stages showed that developing leaves of P. nigrum have more stomata count than the matured leaves. Further analysis with the LI-6400XT Portable Photosynthesis System revealed that photosynthetic stomatal conductance and transpiration rates on the underside (abaxial) of the leaf were significantly higher than on both sides. Information obtained from this study may contribute significantly in subsequent research such as foliar fertiliser application or gas exchange measurements.

Full text available from: Official URL: https://jtpp.org.my/volume/11_1/vol11_1_article4.p...

Abstract

Currently there is little information on whether black pepper leaf has more stomata on the underside above side or the stomata are equally distributed. Thus this paper provides vital information on black pepper leaf stomata distribution and density to shed more light on the subject. The objective of this study was to investigate the distribution pattern of stomata on developing and matured leaf of Piper nigrum var. Kuching Semongok Emas and Semongok Aman. To analyse the leaves we used nail polish staining tools combined with a compound microscope incorporated with imaging software. Our analysis indicated that stomata occur only on the lower side of the leaf (hypostomatic). The result on distribution showed that except for matured leaf of the Kuching variety abaxial stomata were found abundantly at the base of the leaf in all varieties and developmental stages. Stomatal densities according to varieties in P. nigrum leaves recorded that the Semongok Aman has the highest stomata count followed by Semongok Emas and Kuching. Differences in stomatal density between leaves of varying developmental stages showed that developing leaves of P. nigrum have more stomata count than the matured leaves. Further analysis with the LI-6400XT Portable Photosynthesis System revealed that photosynthetic stomatal conductance and transpiration rates on the underside (abaxial) of the leaf were significantly higher than on both sides. Information obtained from this study may contribute significantly in subsequent research such as foliar fertiliser application or gas exchange measurements.

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Item Type:	Article
AGROVOC Term:	Black pepper
AGROVOC Term:	Piper nigrum
AGROVOC Term:	Leaf analysis
AGROVOC Term:	Fields
AGROVOC Term:	Stomata
AGROVOC Term:	Plant developmental stages
AGROVOC Term:	Leaf
AGROVOC Term:	Stomatal conductance
AGROVOC Term:	Photosynthesis
AGROVOC Term:	Transpiration
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8196

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