Isolation and molecular detection of Newcastle Disease virus from an imported meat sample

Leow B. L., . and Basirah M. A., . and Zunaida B., . and Maizatul Z., . and Muhammad R.S., . and Ong G. H., . and Syamsiah A. S., . and Mohd J. R., . and Mohd H. A. H., . and Faizul F. M. Y., . Isolation and molecular detection of Newcastle Disease virus from an imported meat sample. pp. 17-20. ISSN 0128-2506

Infected poultry meat plays an important role in the spread of Newcastle Disease (ND). In this study an imported meat product was found to be positive for ND by both virus isolation and molecular characterization. Analysis of the deduced amino acid sequences of the F protein cleavage site showed that the isolate was virulent as indicated by the sequence 112RRQKR116 for the Cterminus of the F2 protein and phenylanine (F) at the N-terminus of the F1 protein residue 117. Basic Local Alignment Search Tool (BLAST) analysis showed the isolate was 98 identical with China Hebei ND strain. Though the regulations for the importation of poultry meat for human consumption into Malaysia are strict the possibility of the persistence of ND virus in imported meat is prevalent. Strict enforcement of importing regulations and screening the disease in imported poultry meat is important to ensure food safety and prevent introducing ND strain from other countries into Malaysia.

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Abstract

Infected poultry meat plays an important role in the spread of Newcastle Disease (ND). In this study an imported meat product was found to be positive for ND by both virus isolation and molecular characterization. Analysis of the deduced amino acid sequences of the F protein cleavage site showed that the isolate was virulent as indicated by the sequence 112RRQKR116 for the Cterminus of the F2 protein and phenylanine (F) at the N-terminus of the F1 protein residue 117. Basic Local Alignment Search Tool (BLAST) analysis showed the isolate was 98 identical with China Hebei ND strain. Though the regulations for the importation of poultry meat for human consumption into Malaysia are strict the possibility of the persistence of ND virus in imported meat is prevalent. Strict enforcement of importing regulations and screening the disease in imported poultry meat is important to ensure food safety and prevent introducing ND strain from other countries into Malaysia.

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Item Type:	Article
AGROVOC Term:	Newcastle disease
AGROVOC Term:	Poultry meat
AGROVOC Term:	Chicken meat
AGROVOC Term:	Imported breeds
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Virulent pathogens
AGROVOC Term:	Foodborne diseases
AGROVOC Term:	Newcastle disease virus
AGROVOC Term:	Import controls
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8355

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