Molecular detection of Mycoplasma gallisepticum by real time PCR

Yasmin F., . and A. Ideris, . and A. R. Omar, . and M. Hair-Bejo, . and Tan S. W., . and Tan C. G., . and R. Islam, . and K. Ahmad, . Molecular detection of Mycoplasma gallisepticum by real time PCR. pp. 1-7. ISSN 0128-2506

Mycoplasma gallisepticum (MG) causes chronic respiratory disease leading to huge economic losses to the poultry industry worldwide. Early and efficient detection is therefore crucial in reducing the loss sustained by poultry farmers and poultry industry at large. Three main approaches are used for the diagnosis of MG: isolation and identification serology and molecular detection method. Recently real time polymerase chain reaction has been developed for the detection of infectious organisms but so far only a limited number of diagnostic real time PCRs have been proposed for MG. This study was carried out to develop a SYBR green real time PCR assay for the detection of MG using primer set specific to the gapA gene. The primer set was able to amplify the expected DNA fragment of 505 bp. The assay was found to be specific and highly sensitive in detecting MG as indicated by its ability to detect between 260 ng/l to 26 pg/l DNA template. In conclusion this study successfully developed a specific and sensitive real time PCR assay for the rapid detection of MG compared to conventional PCR method. Although the cost to carry out real time PCR is more expensive it is a more specific sensitive and rapid method for detection of MG as compared with conventional PCR.

Full text available from: Official URL: https://www.mavma.org.my/2014-volume-26-issue-no-1...

Abstract

Mycoplasma gallisepticum (MG) causes chronic respiratory disease leading to huge economic losses to the poultry industry worldwide. Early and efficient detection is therefore crucial in reducing the loss sustained by poultry farmers and poultry industry at large. Three main approaches are used for the diagnosis of MG: isolation and identification serology and molecular detection method. Recently real time polymerase chain reaction has been developed for the detection of infectious organisms but so far only a limited number of diagnostic real time PCRs have been proposed for MG. This study was carried out to develop a SYBR green real time PCR assay for the detection of MG using primer set specific to the gapA gene. The primer set was able to amplify the expected DNA fragment of 505 bp. The assay was found to be specific and highly sensitive in detecting MG as indicated by its ability to detect between 260 ng/l to 26 pg/l DNA template. In conclusion this study successfully developed a specific and sensitive real time PCR assay for the rapid detection of MG compared to conventional PCR method. Although the cost to carry out real time PCR is more expensive it is a more specific sensitive and rapid method for detection of MG as compared with conventional PCR.

[error in script]

Item Type:	Article
AGROVOC Term:	Mycoplasma gallisepticum
AGROVOC Term:	Chronic respiratory diseases
AGROVOC Term:	PCR
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Broilers
AGROVOC Term:	Layer chickens
AGROVOC Term:	Chickens
AGROVOC Term:	Isolation techniques
AGROVOC Term:	DNA
AGROVOC Term:	Extraction
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8362

Actions (login required)

View Item