Citation
Umi Salamah Ramli and Abdul Rahim Mohamad (2003) Inhibition studies on lipid synthesis in the oil palm using Cerulenin and 2-Bromooctanoate. [Proceedings Paper]
Abstract
Different approaches of metabolic control analysis were applied to lipid biosynthesis pathway in the oil palm mesocarp to extend our previous findings on carbon flux control. Two separate manipulation experiments using specific inhibitor for fatty acid synthesis (designated as Block A) and triacylglycerol assembly (designated as Block BJ were attempted. Firstly, we manipulated the activity of the fatty acid synthesis reactions using cerulenin and measured the response of the enzymes of the complex lipid assembly reactions, by measuring changes in [1-¹⁴C}acetate incorporation into total lipids, to the resulting changes in the acyl-CoA pool. This data will allow us to calculate group elasticity of the enzymes in Block B in relation to acyl-CoA pool. The second step involved manipulation of enzyme activity within the triacylglycerol assembly pathway by using 2-bromooctanoate. By similar methods, this manipulation will allow us to calculate the group elasticity of Block A in relation to acyl-CoA pool. From these two sets of group elasticities we would be able to calculate the two group flux control coefficients for Block A and Block B, respectively. Initial control experiments were carried out in order to ensure that cerulenin specifically inhibited only Block A. This is important in order to determine whether a specific inhibition by cerulenin on Block A could be obtained and also to confirm that cerulenin had no direct effect on the activity of enzymes in Block B.
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Abstract
Different approaches of metabolic control analysis were applied to lipid biosynthesis pathway in the oil palm mesocarp to extend our previous findings on carbon flux control. Two separate manipulation experiments using specific inhibitor for fatty acid synthesis (designated as Block A) and triacylglycerol assembly (designated as Block BJ were attempted. Firstly, we manipulated the activity of the fatty acid synthesis reactions using cerulenin and measured the response of the enzymes of the complex lipid assembly reactions, by measuring changes in [1-¹⁴C}acetate incorporation into total lipids, to the resulting changes in the acyl-CoA pool. This data will allow us to calculate group elasticity of the enzymes in Block B in relation to acyl-CoA pool. The second step involved manipulation of enzyme activity within the triacylglycerol assembly pathway by using 2-bromooctanoate. By similar methods, this manipulation will allow us to calculate the group elasticity of Block A in relation to acyl-CoA pool. From these two sets of group elasticities we would be able to calculate the two group flux control coefficients for Block A and Block B, respectively. Initial control experiments were carried out in order to ensure that cerulenin specifically inhibited only Block A. This is important in order to determine whether a specific inhibition by cerulenin on Block A could be obtained and also to confirm that cerulenin had no direct effect on the activity of enzymes in Block B.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number |
| AGROVOC Term: | oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis |
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | metabolic profile > metabolic profile Prefer using metabolismmetabolism |
| AGROVOC Term: | experiments > experiments Prefer using experimentationexperimentation |
| AGROVOC Term: | data analysis |
| AGROVOC Term: | plant physiology |
| AGROVOC Term: | biochemists |
| AGROVOC Term: | research scientists > research scientists Prefer using scientistsscientists |
| AGROVOC Term: | metabolic inhibitors > metabolic inhibitors Prefer using antimetabolitesantimetabolites |
| AGROVOC Term: | growth inhibition |
| Geographical Term: | Malaysia |
| Uncontrolled Keywords: | Cerulenin, Bromooctanoate |
| Depositing User: | Nor Hasnita Abdul Samat |
| Date Deposited: | 01 Jul 2024 02:21 |
| Last Modified: | 01 Jul 2024 02:21 |
| URI: | http://webagris.upm.edu.my/id/eprint/838 |
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