Transformation of oil palm with PHB and PHBV gene constructs driven by combination of constitutive promoters

Abdul Masani Mat Yunus and Ho, Chai Ling and Ahmad Parveez Ghulam Kadir (2003) Transformation of oil palm with PHB and PHBV gene constructs driven by combination of constitutive promoters. [Proceedings Paper]

Poly (3-hydroxybutyrate) (PHB) is the most common polyhydroxyalkanoate (PHA) produced as a storage material by bacterial under restricted growth condition. The biosynthetic pathway used by the bacterium A. eutrophus for synthesis of P HE involves three genes product, namely phbA, phbB and phbC, which encodes for thiolase, reductase and synthase enzymes, respectively. Another gene, tdcB, encoding for enzyme threonine dehydratase, is required for producing propionyl CoA, a substrate for the production of a better co-polymer, polyhydroxybutyrate-co-velerate (PHBV). In this study, we have engineered two transformation vectors (pMB26 and pMB27) driven by combination of three different promoters (Ubiquitin, CaMV35S, Actin) for the production of PHB and PHBV in oil palm cells. Transit peptide and matrix attachment region have been incorporated into the vectors for targeting the products into plastids and stabilizing transgene expression, respectively. Transformation of oil palm embryogenic calli and Arabidopsis thaliana floral has been initiated. The efficiency and functionality of the above constructs will be evaluated once Basta TM resistant embryogenic calli and transgenic plants are produced. The progress made to date will be elaborated.

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Abstract

Poly (3-hydroxybutyrate) (PHB) is the most common polyhydroxyalkanoate (PHA) produced as a storage material by bacterial under restricted growth condition. The biosynthetic pathway used by the bacterium A. eutrophus for synthesis of P HE involves three genes product, namely phbA, phbB and phbC, which encodes for thiolase, reductase and synthase enzymes, respectively. Another gene, tdcB, encoding for enzyme threonine dehydratase, is required for producing propionyl CoA, a substrate for the production of a better co-polymer, polyhydroxybutyrate-co-velerate (PHBV). In this study, we have engineered two transformation vectors (pMB26 and pMB27) driven by combination of three different promoters (Ubiquitin, CaMV35S, Actin) for the production of PHB and PHBV in oil palm cells. Transit peptide and matrix attachment region have been incorporated into the vectors for targeting the products into plastids and stabilizing transgene expression, respectively. Transformation of oil palm embryogenic calli and Arabidopsis thaliana floral has been initiated. The efficiency and functionality of the above constructs will be evaluated once Basta TM resistant embryogenic calli and transgenic plants are produced. The progress made to date will be elaborated.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number
AGROVOC Term:	oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis
AGROVOC Term:	genetic transformation
AGROVOC Term:	Gene cloning
AGROVOC Term:	molecular biology
AGROVOC Term:	plasmids
AGROVOC Term:	polymerase chain reaction > polymerase chain reaction Prefer using PCRPCR
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	transgenic plants
AGROVOC Term:	biopolymers
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	23 Jul 2024 02:22
Last Modified:	23 Jul 2024 02:22
URI:	http://webagris.upm.edu.my/id/eprint/843

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