Citation
Halimah Alias and Elyana Md Shariff and Boon, Soo Heong and Tan, Amos Chi Yee and Norsharilah Md Shariff and Wizana Zainal Abidin and Low, Leslie Eng Ti and Rajinder Singh and Wan, Kiew Lian and Tan, Siang Hee and Cheah, Suan Choo (2003) Rapid identification of genes expressed in oil palm (Elaeis guineensis Jaq.) tissue culture. [Proceedings Paper]
Abstract
In an effort to accelerate gene discovery and to understand genes that are expressed during callogenesis and embryogenesis of oil palm tissue culture, ESTs were generated. cDNA libraries were constructed from non-embryogenic callus, embryogenic callus and embryo id tissues. ESTs obtained by random selection of cDNA clones yielded a statistical picture of the level and complexity of genes expressed in the sampled tissues. More than 12,000 5'-end ESTs were generated. Alignment of 10,574 ESTs (PHRED score > 20) allowed the identification of 6,553 unique ESTs of which 5,074 ESTs were present as singletons. Similarity search of the ESTs against a public non-redundant database indicated that about 25% to 50% of the ESTs had significant "hits" to genes in the GenBank database. The putatively identified genes were classified into functional categories. Genes in "protein synthesis" and "metabolism" represent the largest categories. This could reflect the nature of tissues sampled, which are the rapidly growing callus and embryo id stages of oil palm tissue culture.
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Abstract
In an effort to accelerate gene discovery and to understand genes that are expressed during callogenesis and embryogenesis of oil palm tissue culture, ESTs were generated. cDNA libraries were constructed from non-embryogenic callus, embryogenic callus and embryo id tissues. ESTs obtained by random selection of cDNA clones yielded a statistical picture of the level and complexity of genes expressed in the sampled tissues. More than 12,000 5'-end ESTs were generated. Alignment of 10,574 ESTs (PHRED score > 20) allowed the identification of 6,553 unique ESTs of which 5,074 ESTs were present as singletons. Similarity search of the ESTs against a public non-redundant database indicated that about 25% to 50% of the ESTs had significant "hits" to genes in the GenBank database. The putatively identified genes were classified into functional categories. Genes in "protein synthesis" and "metabolism" represent the largest categories. This could reflect the nature of tissues sampled, which are the rapidly growing callus and embryo id stages of oil palm tissue culture.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. TP684 P3I61 2003 Call Number |
| AGROVOC Term: | oil palm > oil palm Prefer using Elaeis guineensisElaeis guineensis |
| AGROVOC Term: | gene expression |
| AGROVOC Term: | tissue culture |
| AGROVOC Term: | molecular biology |
| AGROVOC Term: | genomics |
| AGROVOC Term: | data analysis |
| AGROVOC Term: | plant tissues |
| AGROVOC Term: | plant biotechnology |
| Geographical Term: | Malaysia |
| Depositing User: | Nor Hasnita Abdul Samat |
| Date Deposited: | 03 Aug 2024 06:11 |
| Last Modified: | 03 Aug 2024 06:11 |
| URI: | http://webagris.upm.edu.my/id/eprint/845 |
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