Preliminary study to develop RAPD-DNA fingerprinting in some wild cat species of Peninsular Malaysia

Shabrina S., . and Adura A.M., . and Rahmah M., . Preliminary study to develop RAPD-DNA fingerprinting in some wild cat species of Peninsular Malaysia. pp. 97-103. ISSN 0121-8126

The Random Amplified Polymorphic DNA (RAPD) technique was successfully applied to detect genetic polymorphisms in some wild cat species of Peninsular Malaysia. The animals studied consisted of samples from the Malayan tiger (Panthera tigris) (4) Leopard cat (Prionailurus bengalensis) (4) and Clouded leopard (Pardofelis nebulosa) (1). These animals were analysed with 13 RAPD primers demonstrating the most repeatable amplification patterns. Three primers GEN-1-50-03 GEN-2-50-11 and GEN-1-50-40 generated polymorphic DNA markers specific to P. tigris; GEN-1-50-32 yielded DNA marker characteristic to P. nebulosa and GEN-1-50-44 is specific to P.bengalensis. Preliminary result from this study demonstrates the potential of this technique to detect genetic variations between species as well as within species. Such information is necessary in the formulation of appropriate management strategies directed toward the conservation of these wild cat species.

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Abstract

The Random Amplified Polymorphic DNA (RAPD) technique was successfully applied to detect genetic polymorphisms in some wild cat species of Peninsular Malaysia. The animals studied consisted of samples from the Malayan tiger (Panthera tigris) (4) Leopard cat (Prionailurus bengalensis) (4) and Clouded leopard (Pardofelis nebulosa) (1). These animals were analysed with 13 RAPD primers demonstrating the most repeatable amplification patterns. Three primers GEN-1-50-03 GEN-2-50-11 and GEN-1-50-40 generated polymorphic DNA markers specific to P. tigris; GEN-1-50-32 yielded DNA marker characteristic to P. nebulosa and GEN-1-50-44 is specific to P.bengalensis. Preliminary result from this study demonstrates the potential of this technique to detect genetic variations between species as well as within species. Such information is necessary in the formulation of appropriate management strategies directed toward the conservation of these wild cat species.

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Item Type:	Article
AGROVOC Term:	Tigers
AGROVOC Term:	Leopards
AGROVOC Term:	Genetic variation
AGROVOC Term:	Random amplified polymorphic DNA
AGROVOC Term:	Blood sampling
AGROVOC Term:	Tissue extracts
AGROVOC Term:	DNA fingerprinting
AGROVOC Term:	Genetic differences
AGROVOC Term:	Genetic diversity within species
AGROVOC Term:	Genetic polymorphism
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8460

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