Monoclonal antibodies against the haemagglutinin-Neuraminidase glycoprotein of new castle disease virus strain AF2240.


Citation

Majid Eshaghi, . and Lee L. K., . and Abdul Manaf Ali, . and Khatijah Mohd. Yusoff, . Monoclonal antibodies against the haemagglutinin-Neuraminidase glycoprotein of new castle disease virus strain AF2240. pp. 37-41. ISSN 0128-2506

Abstract

The Malaysian velogenic-viscerotropic Newcastle disease virus (NDV) strain AF2240 is the reference strain that has often been used for vaccine development. Eight hybridoma clones producing monoclonal antibodies (mAbs) against NDV) strain AF2240 were established as a result of fusion between myeloma Sp2/0-Ag14 and Balb/c B- lymphocytes. Isotyping of mAbs showed that clones 1B9 2D6 and 9D7 produced IgGI clones ID5 5A10 and 5F10 produced IgG2a and clones 2G3 and 5E10 produced IgG3. All the mAbs possessed kappa (K) light chain. The mAbs were reacted towards the haemagglutinin-neuraminidase(HN) glycoprotein of the virus and able to inhibit in the haemagglutination with chicken red blood cells but not the haemolysis-inhibition test which confirmed that were specific to the HN glycoprotein and not to the fussion protein.


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Abstract

The Malaysian velogenic-viscerotropic Newcastle disease virus (NDV) strain AF2240 is the reference strain that has often been used for vaccine development. Eight hybridoma clones producing monoclonal antibodies (mAbs) against NDV) strain AF2240 were established as a result of fusion between myeloma Sp2/0-Ag14 and Balb/c B- lymphocytes. Isotyping of mAbs showed that clones 1B9 2D6 and 9D7 produced IgGI clones ID5 5A10 and 5F10 produced IgG2a and clones 2G3 and 5E10 produced IgG3. All the mAbs possessed kappa (K) light chain. The mAbs were reacted towards the haemagglutinin-neuraminidase(HN) glycoprotein of the virus and able to inhibit in the haemagglutination with chicken red blood cells but not the haemolysis-inhibition test which confirmed that were specific to the HN glycoprotein and not to the fussion protein.

Additional Metadata

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Item Type: Article
AGROVOC Term: Glycoproteins
AGROVOC Term: Newcastle disease virus
AGROVOC Term: Screening tests
AGROVOC Term: Indirect ELISA
AGROVOC Term: Haemagglutination inhibition tests
AGROVOC Term: Enzymes
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8613

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