Anti-elastase anti-tyrosinase and antioxidant properties of a peptide fraction obtained from sorghum (Sorghum bicolor L. Moench) grain

MontalvoGonzález E., . and Alcántara-Quintana L. E., . and Ortiz-Basurto R. I., . and Castro-Jácome T. P., . and Lugo-Cervantes E., . and Tovar-Pérez E. G., . Anti-elastase anti-tyrosinase and antioxidant properties of a peptide fraction obtained from sorghum (Sorghum bicolor L. Moench) grain. pp. 1813-1822. ISSN 0116-6514

Nowadays the food industry are developing products that provide benefits to the skin health thus representing a new potential market for the application of biopeptides. The aim of the present work was to investigate the elastase and tyrosinase inhibitory and antioxidant activities of the sorghum kafirins-derived peptide fraction (KAF-P) on human skin cultures exposed to ultraviolet B (UVB) irradiation in vitro. Sorghum kafirins were first hydrolysed with alcalase (degree of hydrolysis 18). For KAF-P separation hydrolysates were fractionated by ultrafiltration with a molecular weight cut-off of 3 kDa. It was observed that exposure to UVB induced overactivity of elastase and tyrosinase as well as decrease in the activity of the antioxidant enzyme system superoxide dismutase (SOD) catalase (CAT) and glutathione peroxidase (GPx). Treatment with KAF-P inhibited elastase and tyrosinase. Moreover KAF-P attenuated the reduction in the activities of SOD CAT and GPx; and maintained or enhanced the activity of these enzymes in normal human skin in vitro. These anti-elastase anti-tyrosinase and antioxidant properties of KAF-P were similar to those obtained with glutathione which is a peptide that plays an important role in protecting skin cells against UVB. The results suggest that KAF-P is able to intervene in the biochemical reactions of the skin and could therefore be considered as a potential candidate for the design of new nutricosmetics and skin-care products.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(06)%202019/18%20-...

Abstract

Nowadays the food industry are developing products that provide benefits to the skin health thus representing a new potential market for the application of biopeptides. The aim of the present work was to investigate the elastase and tyrosinase inhibitory and antioxidant activities of the sorghum kafirins-derived peptide fraction (KAF-P) on human skin cultures exposed to ultraviolet B (UVB) irradiation in vitro. Sorghum kafirins were first hydrolysed with alcalase (degree of hydrolysis 18). For KAF-P separation hydrolysates were fractionated by ultrafiltration with a molecular weight cut-off of 3 kDa. It was observed that exposure to UVB induced overactivity of elastase and tyrosinase as well as decrease in the activity of the antioxidant enzyme system superoxide dismutase (SOD) catalase (CAT) and glutathione peroxidase (GPx). Treatment with KAF-P inhibited elastase and tyrosinase. Moreover KAF-P attenuated the reduction in the activities of SOD CAT and GPx; and maintained or enhanced the activity of these enzymes in normal human skin in vitro. These anti-elastase anti-tyrosinase and antioxidant properties of KAF-P were similar to those obtained with glutathione which is a peptide that plays an important role in protecting skin cells against UVB. The results suggest that KAF-P is able to intervene in the biochemical reactions of the skin and could therefore be considered as a potential candidate for the design of new nutricosmetics and skin-care products.

[error in script]

Item Type:	Article
AGROVOC Term:	Sorghum
AGROVOC Term:	Sorghum bicolor
AGROVOC Term:	Ultrafiltration
AGROVOC Term:	Peptides
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Tyrosinases
AGROVOC Term:	Antioxidants
AGROVOC Term:	Biochemical reactions
AGROVOC Term:	Skins
AGROVOC Term:	Health care
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8763

Actions (login required)

View Item