Detection of chromosome Y-related genetic defect in an infertile cow using polymerase chain reaction

Chee Y. S., . and Abas Mazni O., . and Hawari H., . and Shaharum S., . and A. Sipat, . Detection of chromosome Y-related genetic defect in an infertile cow using polymerase chain reaction. pp. 7-9. ISSN 9128-2506

The zinc-fingger-protein gene was used ad a marker for the Y chromosome in a normal male a normal female and an infertile female cattle. The gene was amplified using polymerase chain reaction (PCR). The PCR product (Zfy) from the genomic DNA of a normal male was a single DNA band of size approximately 440 bp and was cleaved by the restriction enzyme Pst I. The PCR product from the genomic DNA of a normal female was alsi a single DNA band of size approximately 440 bp but this was not cleaved by Pst I. The PCR product from the genomic DNA of the infertile female was found to be a single DNA band of size approximately 440bp and was cleaved by Pst I. thus indicating the presence of the Y chromosome. This may account for the infertility of the animal. The aooroach described here for the diagnosis of y chromosome abnormality in an infertile cow is rapid and more precise compared to the conventional cytological techniques.

Full text available from: Official URL: https://www.mavma.org.my/1995-volume-7-issue-no-1-...

Abstract

The zinc-fingger-protein gene was used ad a marker for the Y chromosome in a normal male a normal female and an infertile female cattle. The gene was amplified using polymerase chain reaction (PCR). The PCR product (Zfy) from the genomic DNA of a normal male was a single DNA band of size approximately 440 bp and was cleaved by the restriction enzyme Pst I. The PCR product from the genomic DNA of a normal female was alsi a single DNA band of size approximately 440 bp but this was not cleaved by Pst I. The PCR product from the genomic DNA of the infertile female was found to be a single DNA band of size approximately 440bp and was cleaved by Pst I. thus indicating the presence of the Y chromosome. This may account for the infertility of the animal. The aooroach described here for the diagnosis of y chromosome abnormality in an infertile cow is rapid and more precise compared to the conventional cytological techniques.

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Item Type:	Article
AGROVOC Term:	Cows
AGROVOC Term:	Infertility
AGROVOC Term:	Sex chromosomes
AGROVOC Term:	Chromosome aberrations
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	DNA
AGROVOC Term:	Genetic abnormalities
AGROVOC Term:	Cytological analysis
AGROVOC Term:	Cytogenetics
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8871

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