Responses of antibody classes and subclasses to equine herpesvirus type-1 (EHV-1) in the serum and respiratory secretions of mice

Azmi M., . Responses of antibody classes and subclasses to equine herpesvirus type-1 (EHV-1) in the serum and respiratory secretions of mice. pp. 15-20. ISSN 9128-2506

A 30-50 mortality was observed in BALB/c mice inoculated with 5 X 10� p. f. u. EHV-1 intranasally. The absence of mortality in surviving mice following re-infection suggested the involvement of acquired protective immunity. This was indicated by a rapid decline of virus titre in the respiratory tissue which was correlated with a rapid increase of IgG antibody detected by means of ELISA. High IgG antibody titre was attributed to its subclass of IgG1. Generally the titre of IgG1 in the serum was closely correlated to those in the upper and lower respiratory tracts. The antibody titre of IgG3 was very low at any time post-infection. The antibody titres of IgG2a and IgG2b were high but rapidly declined upon reaching a peak. IgA antibody titre detected was very low and only in the respiratory secretions. The titre of neutralizing antibody detected was low both in the serum and the respiratory secretions. Based on these results we suggested that IgG1 perhaps IgG2a and IgG2b are the most important antibody subclasses in rendering antibody-mediated protection against EHV-1 infection. In contrast similar protection could not be mediated by neutralizing antibody and IgA antibody.

Full text available from: Official URL: https://www.mavma.org.my/1995-volume-7-issue-no-1-...

Abstract

A 30-50 mortality was observed in BALB/c mice inoculated with 5 X 10� p. f. u. EHV-1 intranasally. The absence of mortality in surviving mice following re-infection suggested the involvement of acquired protective immunity. This was indicated by a rapid decline of virus titre in the respiratory tissue which was correlated with a rapid increase of IgG antibody detected by means of ELISA. High IgG antibody titre was attributed to its subclass of IgG1. Generally the titre of IgG1 in the serum was closely correlated to those in the upper and lower respiratory tracts. The antibody titre of IgG3 was very low at any time post-infection. The antibody titres of IgG2a and IgG2b were high but rapidly declined upon reaching a peak. IgA antibody titre detected was very low and only in the respiratory secretions. The titre of neutralizing antibody detected was low both in the serum and the respiratory secretions. Based on these results we suggested that IgG1 perhaps IgG2a and IgG2b are the most important antibody subclasses in rendering antibody-mediated protection against EHV-1 infection. In contrast similar protection could not be mediated by neutralizing antibody and IgA antibody.

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Item Type:	Article
AGROVOC Term:	Equine herpesvirus
AGROVOC Term:	Horses
AGROVOC Term:	Animal diseases
AGROVOC Term:	Animal viruses
AGROVOC Term:	Disease control
AGROVOC Term:	ELISA
AGROVOC Term:	Antibodies
AGROVOC Term:	Respiratory system
AGROVOC Term:	Immunity
AGROVOC Term:	Infection
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8873

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