Immunisation against equine herpesvirus type-1 (EHV-1) infection in mice: the effect of different antigen preparations and inoculation routes

Azmi M., . Immunisation against equine herpesvirus type-1 (EHV-1) infection in mice: the effect of different antigen preparations and inoculation routes. pp. 47-52. ISSN 9128-2506

The effect of immunization routes and the nature of EHV-1 antigens on immunity against EHV-1 infection was studied. Immunisation via the intravenous (i.v.) intramuscular (i. m.) and intraperitoneal (i.p.) routes with live EHV-1 induced higher IgG antibody response than via the intranasal (i.n.) route. However the formal immunization routes partially or did not result in protection against lethal infection. Partial protection exhibited by reduction in virus titres was limited to the lower respiratory tract only. In contrast despite very poor antibody responses following i. n. immunization the mice were protected against a similar infection. Thus i. n. is suggested to be the most effective route of immunization against EHV-1 infections. In general i. n. immunization using heat- ultraviolet radiation- or formalin-inactivated antigens resulted in higher IgG antibody responses compared to the live virus. Heat-inactivated EHV-1 antigens induced the highest IgG antibody responses with a transient peak. None of these mice however was protected against subsequent lethal infections. The current findings could explain the failure of vaccination of horses using inactivated EHV-1 antigens which are normally given via the i.m. route. It is suggested that effective immunization required live EHV-1 (of less pathogenic strains) as an immunising agent and i. n. as the route of immunization.

Full text available from: Official URL: https://www.mavma.org.my/1995-volume-7-issue-no-2-...

Abstract

The effect of immunization routes and the nature of EHV-1 antigens on immunity against EHV-1 infection was studied. Immunisation via the intravenous (i.v.) intramuscular (i. m.) and intraperitoneal (i.p.) routes with live EHV-1 induced higher IgG antibody response than via the intranasal (i.n.) route. However the formal immunization routes partially or did not result in protection against lethal infection. Partial protection exhibited by reduction in virus titres was limited to the lower respiratory tract only. In contrast despite very poor antibody responses following i. n. immunization the mice were protected against a similar infection. Thus i. n. is suggested to be the most effective route of immunization against EHV-1 infections. In general i. n. immunization using heat- ultraviolet radiation- or formalin-inactivated antigens resulted in higher IgG antibody responses compared to the live virus. Heat-inactivated EHV-1 antigens induced the highest IgG antibody responses with a transient peak. None of these mice however was protected against subsequent lethal infections. The current findings could explain the failure of vaccination of horses using inactivated EHV-1 antigens which are normally given via the i.m. route. It is suggested that effective immunization required live EHV-1 (of less pathogenic strains) as an immunising agent and i. n. as the route of immunization.

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Item Type:	Article
AGROVOC Term:	Equine herpesvirus
AGROVOC Term:	Animal diseases
AGROVOC Term:	Animal viruses
AGROVOC Term:	Infection
AGROVOC Term:	Horses
AGROVOC Term:	Immunization
AGROVOC Term:	Clinical experimentation
AGROVOC Term:	Mice
AGROVOC Term:	Viral antigens
AGROVOC Term:	Antigens
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8878

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