Immunosuppressive effects induced by cyclophosphamide and cyclosporine a in mice infected with equine herpesvirus type-1 (EHV-1)

Azmi M., . Immunosuppressive effects induced by cyclophosphamide and cyclosporine a in mice infected with equine herpesvirus type-1 (EHV-1). pp. 61-66. ISSN 9128-2506

Mice inoculated intranasally with EHV-1 exhibited mild clinical signs of the disease. Severe clinical signs and high mortality (75-100) were observed when mice were treated everyday with cyclosphosphamide (200 mg/kg/day) or cyclosporine A (40 mg/kg/day) and primarily inoculated with 10� PFU. EHV-1. Low IgG antibody titres were detected in the mice treated with cyclosporine A but undetected with cyclophosphamide. In either case virus titres in the respiratory tissues were significantly increased and correlated with the level of viraemia. Prolonged viraemia was noted in the mice treated with cyclosporine A. The mice that were previously immunised with EHV-1 antigens appeared to be more resistant to cyclosporine A than cyclophosphamide. Upon treatment and challenge virus inoculation clinical signs with an increase in virus replication were observed but not the mortality. No. Amnestic IgG antibody response was observed in the mice which were treated with cyclophosphamide. In contrast the antibody titres in cyclosporine A-treated mice were increased but to lower than those in untreated immunised-mice.

Full text available from: Official URL: https://www.mavma.org.my/1995-volume-7-issue-no-2-...

Abstract

Mice inoculated intranasally with EHV-1 exhibited mild clinical signs of the disease. Severe clinical signs and high mortality (75-100) were observed when mice were treated everyday with cyclosphosphamide (200 mg/kg/day) or cyclosporine A (40 mg/kg/day) and primarily inoculated with 10� PFU. EHV-1. Low IgG antibody titres were detected in the mice treated with cyclosporine A but undetected with cyclophosphamide. In either case virus titres in the respiratory tissues were significantly increased and correlated with the level of viraemia. Prolonged viraemia was noted in the mice treated with cyclosporine A. The mice that were previously immunised with EHV-1 antigens appeared to be more resistant to cyclosporine A than cyclophosphamide. Upon treatment and challenge virus inoculation clinical signs with an increase in virus replication were observed but not the mortality. No. Amnestic IgG antibody response was observed in the mice which were treated with cyclophosphamide. In contrast the antibody titres in cyclosporine A-treated mice were increased but to lower than those in untreated immunised-mice.

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Item Type:	Article
AGROVOC Term:	Equine herpesvirus
AGROVOC Term:	Animal diseases
AGROVOC Term:	Animal viruses
AGROVOC Term:	Horses
AGROVOC Term:	Infection
AGROVOC Term:	Immunosuppression
AGROVOC Term:	Clinical experimentation
AGROVOC Term:	Inoculation
AGROVOC Term:	Mice
AGROVOC Term:	Disease treatment
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8879

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