Emulsion-based extraction of -sitosterol and carotenoids from sea buckthorn (Hippophae rhamnoides) pomace


Citation

Tsolmon S., . and Taarji N., . and Tsogtoo B., . and Neves M. A., . and Melanie H., . and Khalid N., . and Kobayashi I., . and Nakajima M., . Emulsion-based extraction of -sitosterol and carotenoids from sea buckthorn (Hippophae rhamnoides) pomace. pp. 56-65. ISSN 2231-7546

Abstract

In the present work we investigated oil-in-water (O/W) emulsion-based extraction of -sitosterol and carotenoids from sea buckthorn pomace. We compared this new green extraction method with conventional extraction using organic solvents and oils. The objective of the present work was to evaluate the efficiency of these different extraction systems on the yields of bioactive compounds from plant-based materials and to determine the optimum extraction conditions for maximum extraction yield. Our results indicated that O/W emulsions prepared without emulsifier using a high-pressure homogeniser had the highest extraction capability for -sitosterol and carotenoids as compared to the other extraction systems. The optimum conditions were 65C for 1 h extraction using emulsifier-free soybean O/W emulsions. Under these conditions the extracted amounts were up to 32.0 mg/g dw (dry weight) for -sitosterol and 1.44 mg/g dw for total carotenoids. The obtained compounds were relatively stable at 5C and 25C for up to 28 days of storage. This emulsion-based extraction method is promising for the extraction of -sitosterol and carotenoids that can be further applied into dietary nutritional supplements and fortified food.


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Abstract

In the present work we investigated oil-in-water (O/W) emulsion-based extraction of -sitosterol and carotenoids from sea buckthorn pomace. We compared this new green extraction method with conventional extraction using organic solvents and oils. The objective of the present work was to evaluate the efficiency of these different extraction systems on the yields of bioactive compounds from plant-based materials and to determine the optimum extraction conditions for maximum extraction yield. Our results indicated that O/W emulsions prepared without emulsifier using a high-pressure homogeniser had the highest extraction capability for -sitosterol and carotenoids as compared to the other extraction systems. The optimum conditions were 65C for 1 h extraction using emulsifier-free soybean O/W emulsions. Under these conditions the extracted amounts were up to 32.0 mg/g dw (dry weight) for -sitosterol and 1.44 mg/g dw for total carotenoids. The obtained compounds were relatively stable at 5C and 25C for up to 28 days of storage. This emulsion-based extraction method is promising for the extraction of -sitosterol and carotenoids that can be further applied into dietary nutritional supplements and fortified food.

Additional Metadata

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Item Type: Article
AGROVOC Term: Sea buckthorn
AGROVOC Term: Pomacea
AGROVOC Term: Emulsions
AGROVOC Term: Extraction
AGROVOC Term: Sitosterol
AGROVOC Term: Carotenoids
AGROVOC Term: Chemical composition
AGROVOC Term: Biological properties
AGROVOC Term: Peel
AGROVOC Term: Seeds
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8972

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