Antioxidant antimicrobial and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts

Savcı A., . and Koçpınar E. F., . and Alan Y., . and Kurşat M., . Antioxidant antimicrobial and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. pp. 160-170. ISSN 2231-7546

Tanacetum species have been used traditionally for a long time due to their various biological activities such as anticoagulant antimicrobial cytotoxic insecticidal and antimigraine. In the present work ethanolic extracts were prepared from aerial parts of four Tanacetum species (T. parthenifolium T. chiliophyllum T. balsamitoides and T. zahlbruckneri) by Soxhlet Extraction Method; and 17 different phenolic concentrations of the extracts were determined by HPLC. Subsequently antimicrobial effects were investigated with hollow agar method and in vitro antioxidant activities including total antioxidant activity DPPH (2 2-diphenyl-1-picrylhydrazyl) ABTS (2 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity ferric iron (Fe�) and cupric (Cu�) reduction. Finally protective effects on pBR322 plasmid DNA damaged by H‚‚O‚‚ using gel electrophoresis were investigated. Although a few phenolics were well known as antioxidant it could not be detected in some of the extracts. The plant extracts however were generally found to have high phenolic content. In the antimicrobial studies extracts of T. parthenifolium and T. zahlbruckneri did not have a lethal effect on S. cerevisiae. The extracts were also found to have powerful antioxidant properties. They exhibited similar activity with the standards which are butylated hydroxyanisole (BHA) butylated hydroxytoluene (BHT) and -tocopherol (-Toc). In addition they did not show useful effect on the damages of H‚‚O‚‚ and/or DMSO depending on the concentration. However when extracts were applied alone they partly stabilised the plasmid DNA. It was also observed that the high phenolic content of T. chiliophyllum extract had a positive effect on its antioxidant activity and DNA protective activity.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(01)%202020/17%20-...

Abstract

Tanacetum species have been used traditionally for a long time due to their various biological activities such as anticoagulant antimicrobial cytotoxic insecticidal and antimigraine. In the present work ethanolic extracts were prepared from aerial parts of four Tanacetum species (T. parthenifolium T. chiliophyllum T. balsamitoides and T. zahlbruckneri) by Soxhlet Extraction Method; and 17 different phenolic concentrations of the extracts were determined by HPLC. Subsequently antimicrobial effects were investigated with hollow agar method and in vitro antioxidant activities including total antioxidant activity DPPH (2 2-diphenyl-1-picrylhydrazyl) ABTS (2 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity ferric iron (Fe�) and cupric (Cu�) reduction. Finally protective effects on pBR322 plasmid DNA damaged by H‚‚O‚‚ using gel electrophoresis were investigated. Although a few phenolics were well known as antioxidant it could not be detected in some of the extracts. The plant extracts however were generally found to have high phenolic content. In the antimicrobial studies extracts of T. parthenifolium and T. zahlbruckneri did not have a lethal effect on S. cerevisiae. The extracts were also found to have powerful antioxidant properties. They exhibited similar activity with the standards which are butylated hydroxyanisole (BHA) butylated hydroxytoluene (BHT) and -tocopherol (-Toc). In addition they did not show useful effect on the damages of H‚‚O‚‚ and/or DMSO depending on the concentration. However when extracts were applied alone they partly stabilised the plasmid DNA. It was also observed that the high phenolic content of T. chiliophyllum extract had a positive effect on its antioxidant activity and DNA protective activity.

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Item Type:	Article
AGROVOC Term:	Tanacetum
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Antioxidants
AGROVOC Term:	DNA
AGROVOC Term:	Species
AGROVOC Term:	Ethanol
AGROVOC Term:	Plant extracts
AGROVOC Term:	Plant collections
AGROVOC Term:	HPLC
AGROVOC Term:	Laboratory experimentation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8983

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