Plasmid and antimicrobial resistance transfer from poultry strains to aquatic strains of Escherichia coli

Son R., . and Rusul G, . and M. I. A. Karim, . Plasmid and antimicrobial resistance transfer from poultry strains to aquatic strains of Escherichia coli. pp. 7-13. ISSN 9128-2506

Plasmid DNA analysis and antibiotic susceptibility were used to study strains of Escherichia coli isolated from poultry and aquatic sources. Nineteen of 25 and 22 of 30 among the aquatic and poultry strains respectively were found to contain plasmid DNA bands ranging in size from 2.3 to 102 kilobases (kb). Resistances to five or more of the antimicrobial agents tested were predominant among the poultry strains. However none were susceptible to all antimicrobial agents tested. More isolates containing plasmid were resistant to ampicillin erythromycin streptomycin and tetracycline. The potential of the transfer of nature plasmids between poultry and aquatic strains has been studied. Four antibiotic-multiresistant poultry isolates (EP201 EP205 EP210 amd EP211) possessing potentially conjugative large plasmids were mated with two aquatic isolate (EA101 and EA106) and a laboratory strain E. Coli K12 C600 Na in sterilised river water at 37 C and 25 C. and LB medium at 37C. Conjugation occurred in all system tested with en bloc transfer of the donors antibiotic resistance and plasmid DNA bands. In invitro transfer conducted at 25C in sterile river water transfer frequency values decreased by 2 to 3 logs from the optimum conditions at 37C.

Full text available from: Official URL: https://storage.unitedwebnetwork.com/files/478/8d1...

Abstract

Plasmid DNA analysis and antibiotic susceptibility were used to study strains of Escherichia coli isolated from poultry and aquatic sources. Nineteen of 25 and 22 of 30 among the aquatic and poultry strains respectively were found to contain plasmid DNA bands ranging in size from 2.3 to 102 kilobases (kb). Resistances to five or more of the antimicrobial agents tested were predominant among the poultry strains. However none were susceptible to all antimicrobial agents tested. More isolates containing plasmid were resistant to ampicillin erythromycin streptomycin and tetracycline. The potential of the transfer of nature plasmids between poultry and aquatic strains has been studied. Four antibiotic-multiresistant poultry isolates (EP201 EP205 EP210 amd EP211) possessing potentially conjugative large plasmids were mated with two aquatic isolate (EA101 and EA106) and a laboratory strain E. Coli K12 C600 Na in sterilised river water at 37 C and 25 C. and LB medium at 37C. Conjugation occurred in all system tested with en bloc transfer of the donors antibiotic resistance and plasmid DNA bands. In invitro transfer conducted at 25C in sterile river water transfer frequency values decreased by 2 to 3 logs from the optimum conditions at 37C.

[error in script]

Item Type:	Article
AGROVOC Term:	Plasmids
AGROVOC Term:	Antimicrobial resistance
AGROVOC Term:	Poultry
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Sampling
AGROVOC Term:	Data collecting
AGROVOC Term:	Gel electrophoresis
AGROVOC Term:	Laboratory experimentation
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Epidemiology
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9038

Actions (login required)

View Item