A modified Folin-Ciocalteu method for the microdetermination of total phenolic content in honey

Bai W. S., . and Zhang J. J., . and Cheng N., . and Cao W., . and Liu C. Y., . A modified Folin-Ciocalteu method for the microdetermination of total phenolic content in honey. pp. 576-584. ISSN 2231-7546

Polyphenols are major antioxidant activity-associated bioactive substances in honey. Measurement of the total phenolic content in honey is of great importance for predicting antioxidant activity and characterising or selecting honey samples for their use as antioxidants or natural food preservatives. Considering that the original Folin-Ciocalteu (F-C) assay for total phenolic content determination is either time-consuming or high-cost a microplate reader-based study was conducted to modify and validate the original F-C assay for the microdetermination of total phenolic content in honey samples. The linearity (R 0.9982) detection limit (8.61 0.33 mg gallic acid equivalents (GAE)kg- honey) quantitation limit (26.08 0.99 mg GAEkg- honey) and recovery rate (93.46 - 109.99) were acceptable. The results suggested that the proposed F-C assay is fast sensitive precise and repeatable. The method was successfully applied to analyse a total of 33 honey samples from 9 floral origins and 12 geographical regions. The modified F-C assay is low cost and easy to operate and therefore could be applied to routine analysis.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(03)%202020/DONE%2...

Abstract

Polyphenols are major antioxidant activity-associated bioactive substances in honey. Measurement of the total phenolic content in honey is of great importance for predicting antioxidant activity and characterising or selecting honey samples for their use as antioxidants or natural food preservatives. Considering that the original Folin-Ciocalteu (F-C) assay for total phenolic content determination is either time-consuming or high-cost a microplate reader-based study was conducted to modify and validate the original F-C assay for the microdetermination of total phenolic content in honey samples. The linearity (R 0.9982) detection limit (8.61 0.33 mg gallic acid equivalents (GAE)kg- honey) quantitation limit (26.08 0.99 mg GAEkg- honey) and recovery rate (93.46 - 109.99) were acceptable. The results suggested that the proposed F-C assay is fast sensitive precise and repeatable. The method was successfully applied to analyse a total of 33 honey samples from 9 floral origins and 12 geographical regions. The modified F-C assay is low cost and easy to operate and therefore could be applied to routine analysis.

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Item Type:	Article
AGROVOC Term:	Honey
AGROVOC Term:	Determination of chemicals
AGROVOC Term:	Bioassays
AGROVOC Term:	Analytical methods
AGROVOC Term:	Phenolic content
AGROVOC Term:	Gallic acid
AGROVOC Term:	Antioxidants
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9157

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