Green synthesis of silver nanoparticles using banana flower extract, and their antibacterial activity

Weiming, G. and Quanfeng, H. and Jianxia, S. and Dan, L. and Xuejuan, D. (2023) Green synthesis of silver nanoparticles using banana flower extract, and their antibacterial activity. International Food Research Journal (Malaysia), 30. pp. 613-625. ISSN 2231-7546

Silver nanoparticles (AgNPs) were synthesised using banana flower extract (BFE) as a reducing and stabilising agent. Spherical, well-dispersed, and stable AgNPs were formed and characterised by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), and zeta potential. The in vitro antimicrobial properties of AgNPs against Staphylococcus aureus and Escherichia coli were then investigated. The minimum inhibitory concentration (MIC) of AgNPs against S. aureus and E. coli were 32 and 16 μg/mL, respectively. E. coli was more sensitive to AgNPs than S. aureus due to differences in cell wall structures of the bacteria. Regarding the bactericidal mechanisms of AgNPs, an increase in cell permeability and a distinctive deformation in cellular morphology was observed. The antibacterial effect decreased with the addition of the antioxidant N-acetyl-l-cysteine (NAC) which acted as ROS scavenger. In summary, the antibacterial mechanism was likely a combination of cell membrane damage and ROS induction.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(03)%202023/06%20-...

Abstract

Silver nanoparticles (AgNPs) were synthesised using banana flower extract (BFE) as a reducing and stabilising agent. Spherical, well-dispersed, and stable AgNPs were formed and characterised by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), and zeta potential. The in vitro antimicrobial properties of AgNPs against Staphylococcus aureus and Escherichia coli were then investigated. The minimum inhibitory concentration (MIC) of AgNPs against S. aureus and E. coli were 32 and 16 μg/mL, respectively. E. coli was more sensitive to AgNPs than S. aureus due to differences in cell wall structures of the bacteria. Regarding the bactericidal mechanisms of AgNPs, an increase in cell permeability and a distinctive deformation in cellular morphology was observed. The antibacterial effect decreased with the addition of the antioxidant N-acetyl-l-cysteine (NAC) which acted as ROS scavenger. In summary, the antibacterial mechanism was likely a combination of cell membrane damage and ROS induction.

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Item Type:	Article
AGROVOC Term:	flower extracts
AGROVOC Term:	nanoparticles
AGROVOC Term:	biosynthesis
AGROVOC Term:	sampling
AGROVOC Term:	plant extracts
AGROVOC Term:	sustainable agriculture
Geographical Term:	China
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	28 Jul 2024 14:53
Last Modified:	28 Jul 2024 14:54
URI:	http://webagris.upm.edu.my/id/eprint/924

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