Genetic diversity of endangered snakehead Channa lucius (Cuvier 1831) in the Mekong Delta inferred from ISSR markers


Citation

Sawasawa Wilson, . and Duong Thuy-Yen, . Genetic diversity of endangered snakehead Channa lucius (Cuvier 1831) in the Mekong Delta inferred from ISSR markers. pp. 266-273. ISSN 0116-6514

Abstract

Channa lucius (Cuvier 1831) is one of the endangered and economically important species in the Channidae family with high potential for aquaculture in the Mekong Delta. Limited genetic information has hindered the conservation and domestication programs of this species. The present study used inter-simple sequence repeats (ISSR) markers to assess the genetic diversity of C. lucius. Samples were collected from three conservation locations (Ca Mau Kien Giang and Long An) and one from inland fisheries areas (Can Tho) in the Mekong Delta. Eight highly polymorphic markers yielded 76 scorable bands from 93 samples (22 to 24 samples per population) with the size range from 450 to 2000 bp. Results revealed high levels of genetic diversity of C. lucius populations with unbiased expected heterozygosity from 0.250 to 0.271 and Shannon index from 0.364 to 0.395 in which those from Ca Mau population were lowest. Neis genetic distance between populations ranged from 0.022 to 0.057 with low overall genetic difference GST 0.090 and high gene flow (Nm 5.06) indicating that C. lucius populations were not subdivided. This study provides useful information for conservation projects of C. lucius in the region.


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Abstract

Channa lucius (Cuvier 1831) is one of the endangered and economically important species in the Channidae family with high potential for aquaculture in the Mekong Delta. Limited genetic information has hindered the conservation and domestication programs of this species. The present study used inter-simple sequence repeats (ISSR) markers to assess the genetic diversity of C. lucius. Samples were collected from three conservation locations (Ca Mau Kien Giang and Long An) and one from inland fisheries areas (Can Tho) in the Mekong Delta. Eight highly polymorphic markers yielded 76 scorable bands from 93 samples (22 to 24 samples per population) with the size range from 450 to 2000 bp. Results revealed high levels of genetic diversity of C. lucius populations with unbiased expected heterozygosity from 0.250 to 0.271 and Shannon index from 0.364 to 0.395 in which those from Ca Mau population were lowest. Neis genetic distance between populations ranged from 0.022 to 0.057 with low overall genetic difference GST 0.090 and high gene flow (Nm 5.06) indicating that C. lucius populations were not subdivided. This study provides useful information for conservation projects of C. lucius in the region.

Additional Metadata

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Item Type: Article
AGROVOC Term: Channa
AGROVOC Term: Channidae
AGROVOC Term: Fishes
AGROVOC Term: Fisheries
AGROVOC Term: Aquaculture
AGROVOC Term: Genetic markers
AGROVOC Term: Sampling
AGROVOC Term: DNA
AGROVOC Term: Extraction
AGROVOC Term: Electrophoresis
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/9295

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