Comparative genomic analysis and proteolytic properties of Lactobacillus curieae CCTCC M2011381

Chen Y., . and Xie J., . and Sun J., . and Zhou Z., . and Zhang R., . and Li H., . and Wei D., . Comparative genomic analysis and proteolytic properties of Lactobacillus curieae CCTCC M2011381. pp. 883-892. ISSN 22317546

Lactobacillus curieae CCTCC M2011381 a novel strain initially isolated from the brine of stinky tofu acts as a starter in soymilk but not in milk. We investigated how evolution and physiology help L. curieae adapt to the soy environment. To do so its whole genome was obtained through a large-scale sequencing using Illumina HiSeq 4000 and PacBio RSII. A comparative genome analysis between the strain and other four non-starter dairy strains namely Lactobacillus brevis ATCC 367 Pediococcus pentosaceus ATCC 25745 L. plantarum WCFS1 and L. reuteri JCM 1112 was carried out based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups (COG) of protein databases. The results indicated that the assembled genome comprised one circular chromosome (2.1 Mb) without a plasmid. The strain was not only phylogenetically close to L. brevis ATCC 367 but also had a close COG distribution to L. brevis ATCC 367. The strain lacked cell envelope proteinase (CEP) which is used for the initiation of casein utilisation and some oligopeptide transporter systems for the di- tri- and tetrapeptides (Dpp system) as detected by rapid annotation using the Subsystem Technology annotation sever. However it did have an oligopeptide transporter (Opp) system a peptidolysis system and an amino acid synthetic pathway. The present work provides insight into the evolution of the L. curieae M2011381 proteolytic system and its application in the fermentation of soy material.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(05)%202020/DONE%2...

Abstract

Lactobacillus curieae CCTCC M2011381 a novel strain initially isolated from the brine of stinky tofu acts as a starter in soymilk but not in milk. We investigated how evolution and physiology help L. curieae adapt to the soy environment. To do so its whole genome was obtained through a large-scale sequencing using Illumina HiSeq 4000 and PacBio RSII. A comparative genome analysis between the strain and other four non-starter dairy strains namely Lactobacillus brevis ATCC 367 Pediococcus pentosaceus ATCC 25745 L. plantarum WCFS1 and L. reuteri JCM 1112 was carried out based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups (COG) of protein databases. The results indicated that the assembled genome comprised one circular chromosome (2.1 Mb) without a plasmid. The strain was not only phylogenetically close to L. brevis ATCC 367 but also had a close COG distribution to L. brevis ATCC 367. The strain lacked cell envelope proteinase (CEP) which is used for the initiation of casein utilisation and some oligopeptide transporter systems for the di- tri- and tetrapeptides (Dpp system) as detected by rapid annotation using the Subsystem Technology annotation sever. However it did have an oligopeptide transporter (Opp) system a peptidolysis system and an amino acid synthetic pathway. The present work provides insight into the evolution of the L. curieae M2011381 proteolytic system and its application in the fermentation of soy material.

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Item Type:	Article
AGROVOC Term:	Lactobacillus
AGROVOC Term:	Lactobacillus brevis
AGROVOC Term:	Pediococcus pentosaceus
AGROVOC Term:	Lactobacillus plantarum
AGROVOC Term:	Genomes
AGROVOC Term:	Enzymes
AGROVOC Term:	Fermentation
AGROVOC Term:	Proteolysis
AGROVOC Term:	Proteinases
AGROVOC Term:	Oligopeptides
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9404

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