Diversity of vibrio species and their antibiotic resistance patterns in black tiger shrimp Penaeus monodon fabricius 1798 cultured in South-West Region of Bangladesh

Abedin Md. Minhazul, . and Eshik Md Mostavi Enan, . and Begum Mst Khadiza, . and Rahman Mohammad Shamsur, . and Punom Nusrat Jahan, . Diversity of vibrio species and their antibiotic resistance patterns in black tiger shrimp Penaeus monodon fabricius 1798 cultured in South-West Region of Bangladesh. pp. 330-340. ISSN 0116-6514

Shrimp aquaculture industry comprises 15 of the internationally traded seafood products targeted for human consumption. Diseases of shrimp have caused severe economic losses to the aquaculture industry. Among pathogenic bacteria several Vibrio species known as shrimp pathogens are also human pathogens. This study was conducted to identify the incidence of diversified Vibrio species in the shrimp Penaeus monodon Fabricius 1798 farms in Bangladesh. Different Vibrio selective media were used for primary isolation and 16S rRNA gene amplicons were sequenced followed by phylogenetic analysis. The Vibrio strains were also tested against twelve antibiotics. Two strains V32 and V38 showed 99.93 similarity with Vibrio azureus whereas V9 and V33 showed 100 identity with Vibrio fluvialis. Comparison of 16S rRNA gene sequence of V37 with sequences from GenBank identified the strain as Vibrio xuii. The phylogenetic tree indicates that V24 V26 V34 and V35 are closely related with Vibrio parahaemolyticus. The strains V28 and V31 were identified as Vibrio vulnificus. One strain of Vibrio cholerae was also identified. Moreover V22 was identified as Photobacterium damselae subsp. damselae and V25 as Aeromonas hydrophila. Twelve strains of Vibrio were resistant to amoxycillin whereas all 16 bacterial isolates showed sensitivity to nitrofurantoin sulphamethoxazole chloramphenicol ciprofloxacin and tetracycline. Multidrug resistance was observed in three isolates viz. V28 V32 and V35 which were identified as V. vulnificus V. azureus and V. parahaemolyticus respectively having MAR index of 0.25. These findings might have potential applications in controlling shrimp and human pathogenic vibrios in the farming regions.

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Abstract

Shrimp aquaculture industry comprises 15 of the internationally traded seafood products targeted for human consumption. Diseases of shrimp have caused severe economic losses to the aquaculture industry. Among pathogenic bacteria several Vibrio species known as shrimp pathogens are also human pathogens. This study was conducted to identify the incidence of diversified Vibrio species in the shrimp Penaeus monodon Fabricius 1798 farms in Bangladesh. Different Vibrio selective media were used for primary isolation and 16S rRNA gene amplicons were sequenced followed by phylogenetic analysis. The Vibrio strains were also tested against twelve antibiotics. Two strains V32 and V38 showed 99.93 similarity with Vibrio azureus whereas V9 and V33 showed 100 identity with Vibrio fluvialis. Comparison of 16S rRNA gene sequence of V37 with sequences from GenBank identified the strain as Vibrio xuii. The phylogenetic tree indicates that V24 V26 V34 and V35 are closely related with Vibrio parahaemolyticus. The strains V28 and V31 were identified as Vibrio vulnificus. One strain of Vibrio cholerae was also identified. Moreover V22 was identified as Photobacterium damselae subsp. damselae and V25 as Aeromonas hydrophila. Twelve strains of Vibrio were resistant to amoxycillin whereas all 16 bacterial isolates showed sensitivity to nitrofurantoin sulphamethoxazole chloramphenicol ciprofloxacin and tetracycline. Multidrug resistance was observed in three isolates viz. V28 V32 and V35 which were identified as V. vulnificus V. azureus and V. parahaemolyticus respectively having MAR index of 0.25. These findings might have potential applications in controlling shrimp and human pathogenic vibrios in the farming regions.

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Item Type:	Article
AGROVOC Term:	Prawns and shrimps
AGROVOC Term:	Penaeus monodon
AGROVOC Term:	Vibriosis
AGROVOC Term:	Animal diseases
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Antibiotic resistance
AGROVOC Term:	Antimicrobial resistance
AGROVOC Term:	Disease prevention
AGROVOC Term:	Aquaculture
AGROVOC Term:	Animal production
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9470

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