Influence of extraction solvents on total phenolic content and antioxidant activity of Passiflora edulis Sims extract

Muhammad Izzat Zakwan Samudin and Muhammad Yusran Abdul Aziz and Nurhayati Yusof (2022) Influence of extraction solvents on total phenolic content and antioxidant activity of Passiflora edulis Sims extract. Journal of Agrobiotechnology (Malaysia), 13. pp. 1-9. ISSN 2180-1983

Passion fruit is a type of wild exotic plant in Malaysia. Common passion fruits planted in Malaysia are Passiflora edulisSims (purple) and Passiflora edulis var flavicarpa(yellow). These fruits contain a high amount of antioxidants, polyphenols and vitamin C and are widely used as fruit juice, jam, and syrup product. However, there is a lack of information about the nutritional composition of the juice and seed of P. edulis (purple) cultivated in Malaysia. This study aims to determine the total phenolic contents,antioxidant activity and Vitamin C (ascorbic acid) content of P. edulis (purple) using two different solvent extraction,70% of ethanol and 70% of methanol. The total phenolic content (TPC) was carried out by using Folin-Ciocalteu (FC) method, while the antioxidant activity was analysedusing 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay to determine the IC50value of extracted samples. Ascorbic acid content from fresh juice and seeds samples wereanalysed by using Indophenol (DCPIP) method. The resultshowedthat the extraction using ethanol produced the highest TPC with 9.249±0.04mg GAE/g in seeds and 0.0139±0.02mg GAE/g in juice, respectively. The lowest IC₅ₒ value was obtained by a sample extracted with ethanol,which was 83.85±4.66 mg/L in seed and 305.47±5.28 mg/L in juice. The seed sample (53.68±4.95 mg/100mg) exhibited a high ascorbic acid content compared to the juice sample(46.66±3.28mg/100mg). In conclusion, seeds of P. edulis possessed the strongest antioxidant activity and vitamin C content. This study could contribute to information regarding the phytochemical constituents and suitable solvents for extraction of phenolic compounds that can act as antioxidants and thus promote health effects on the human body

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Abstract

Passion fruit is a type of wild exotic plant in Malaysia. Common passion fruits planted in Malaysia are Passiflora edulisSims (purple) and Passiflora edulis var flavicarpa(yellow). These fruits contain a high amount of antioxidants, polyphenols and vitamin C and are widely used as fruit juice, jam, and syrup product. However, there is a lack of information about the nutritional composition of the juice and seed of P. edulis (purple) cultivated in Malaysia. This study aims to determine the total phenolic contents,antioxidant activity and Vitamin C (ascorbic acid) content of P. edulis (purple) using two different solvent extraction,70% of ethanol and 70% of methanol. The total phenolic content (TPC) was carried out by using Folin-Ciocalteu (FC) method, while the antioxidant activity was analysedusing 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay to determine the IC50value of extracted samples. Ascorbic acid content from fresh juice and seeds samples wereanalysed by using Indophenol (DCPIP) method. The resultshowedthat the extraction using ethanol produced the highest TPC with 9.249±0.04mg GAE/g in seeds and 0.0139±0.02mg GAE/g in juice, respectively. The lowest IC₅ₒ value was obtained by a sample extracted with ethanol,which was 83.85±4.66 mg/L in seed and 305.47±5.28 mg/L in juice. The seed sample (53.68±4.95 mg/100mg) exhibited a high ascorbic acid content compared to the juice sample(46.66±3.28mg/100mg). In conclusion, seeds of P. edulis possessed the strongest antioxidant activity and vitamin C content. This study could contribute to information regarding the phytochemical constituents and suitable solvents for extraction of phenolic compounds that can act as antioxidants and thus promote health effects on the human body

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Item Type:	Article
AGROVOC Term:	Passiflora edulis
AGROVOC Term:	passion fruits
AGROVOC Term:	plant extracts
AGROVOC Term:	phenolic compounds
AGROVOC Term:	solvents
AGROVOC Term:	sampling
AGROVOC Term:	statistical analysis > statistical analysis Prefer using statistical methodsstatistical methods
AGROVOC Term:	bioactive compounds
AGROVOC Term:	quality control
AGROVOC Term:	yield increases
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	27 Aug 2024 08:04
Last Modified:	27 Aug 2024 08:04
URI:	http://webagris.upm.edu.my/id/eprint/951

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