Transcriptomics of microdissected staminodes and early developing carpels from female inflorescences of Elaeis guineensis

Azimi Nuraziyan, . and Norashikin Sarpan, . and Abu Bakar Nor-Azwani, . and Ooi Siew Eng, . and Norazlin Abdul Aziz, . and Meilina Ong-Abdullah, . Transcriptomics of microdissected staminodes and early developing carpels from female inflorescences of Elaeis guineensis. pp. 559-568. ISSN 1511-2780

In an abnormal female inflorescence from a mantled clonal palm the male reproductive organs develop into pseudocarpels. Transcriptomes of microdissected male and female reproductive organs in a normal inflorescence were thus compared to identify genes that were differentially expressed during the normal development of these two reproductive organs. Besides the increased expression of stamen identity MADS- box genes EgDEF1 EgGLO1 and EgGLO2 in male reproductive organs NAC and homeodomain leucine- zipper type of transcription factors were also upregulated. Male reproductive organ development may be associated with increased ethylene and decreased bioactive gibberellin levels inferred from the increased expression levels of ACC OXIDASE and GA 2-OXIDASE genes. This expression pattern may be important for the impending developmental arrest of the staminodes as they do not develop into stamens. Early carpel development occurred in conjunction with increased expression of the floral meristem identity LEAFY and organ boundary specification CUP-SHAPED COTYLEDON 2 (CUC2) genes. Hence genes important for male reproductive organ development and early carpel development were identified using precise isolation of specific reproductive organs.

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Abstract

In an abnormal female inflorescence from a mantled clonal palm the male reproductive organs develop into pseudocarpels. Transcriptomes of microdissected male and female reproductive organs in a normal inflorescence were thus compared to identify genes that were differentially expressed during the normal development of these two reproductive organs. Besides the increased expression of stamen identity MADS- box genes EgDEF1 EgGLO1 and EgGLO2 in male reproductive organs NAC and homeodomain leucine- zipper type of transcription factors were also upregulated. Male reproductive organ development may be associated with increased ethylene and decreased bioactive gibberellin levels inferred from the increased expression levels of ACC OXIDASE and GA 2-OXIDASE genes. This expression pattern may be important for the impending developmental arrest of the staminodes as they do not develop into stamens. Early carpel development occurred in conjunction with increased expression of the floral meristem identity LEAFY and organ boundary specification CUP-SHAPED COTYLEDON 2 (CUC2) genes. Hence genes important for male reproductive organ development and early carpel development were identified using precise isolation of specific reproductive organs.

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Item Type:	Article
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Inflorescences
AGROVOC Term:	Female plants
AGROVOC Term:	Carpels
AGROVOC Term:	RNA sequence
AGROVOC Term:	Floral initiation
AGROVOC Term:	Ethylene
AGROVOC Term:	Gibberellins
AGROVOC Term:	Plant reproductive organs
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9511

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