Improvement of Bioethanol Production in Consolidated Bioprocessing (CBP) via Consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581


Citation

Mona Fatin Syazwanee Mohamed Ghazali, . and Muskhazli Mustafa, . and Nur Ain Izzati Mohd Zainudin, . and Nor Azwady Abd Aziz, . Improvement of Bioethanol Production in Consolidated Bioprocessing (CBP) via Consortium of Aspergillus niger B2484 and Trichoderma asperellum B1581. pp. 301-316. ISSN 2231-8526

Abstract

Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However the main disadvantage of this process is the complexity to optimise all factors considering both enzymes and microbial activity at the same time. Hence this study aimed to optimize suitable culture conditions for both organisms to work efficiently. Six single factors that are considered crucial for bioethanol production were tested in one-factor-at-a-time (OFAT) analysis and analysed using Response Surface Methodology (RSM) software for Aspergillus niger B2484 and Trichoderma asperellum B1581 strains. The formulation of a new consortia setting was developed based on the average of two settings generated from RSM testing several combinations of consortia concentrations (5:1 2:4 3:3 4:2 and 1:5). The combination of 5:1 Aspergillus niger B2484 and Trichoderma asperellum B1581 produced the most ethanol with 1.03 g/L more than A. niger B2484 alone with 0.34 g/L of ethanol indicating the potential of the combination of A. niger B2484 and T. asperellum B1581 co-culture for bioethanol production in CBP.


Download File

Full text available from:

Abstract

Consolidated bioprocessing (CBP) in bioethanol production involves the combination of four essential biological procedures in a single bioreactor using a mixture of organisms with favourable cellulolytic ability without the addition of exogenous enzymes. However the main disadvantage of this process is the complexity to optimise all factors considering both enzymes and microbial activity at the same time. Hence this study aimed to optimize suitable culture conditions for both organisms to work efficiently. Six single factors that are considered crucial for bioethanol production were tested in one-factor-at-a-time (OFAT) analysis and analysed using Response Surface Methodology (RSM) software for Aspergillus niger B2484 and Trichoderma asperellum B1581 strains. The formulation of a new consortia setting was developed based on the average of two settings generated from RSM testing several combinations of consortia concentrations (5:1 2:4 3:3 4:2 and 1:5). The combination of 5:1 Aspergillus niger B2484 and Trichoderma asperellum B1581 produced the most ethanol with 1.03 g/L more than A. niger B2484 alone with 0.34 g/L of ethanol indicating the potential of the combination of A. niger B2484 and T. asperellum B1581 co-culture for bioethanol production in CBP.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Ethanol
AGROVOC Term: Ethyl alcohol
AGROVOC Term: Biological production
AGROVOC Term: Fermentation
AGROVOC Term: Aspergillus niger
AGROVOC Term: Trichoderma
AGROVOC Term: Fungi
AGROVOC Term: Temperature
AGROVOC Term: Enzymes
AGROVOC Term: Enzyme activity
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/9517

Actions (login required)

View Item View Item
Agris UPM is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.