The diversity of halotolerant and halophilic bacteria in the soil of the Nasinuan Secondary Forest in Maha Sarakham Thailand

Thalisa Yuwa-Amornpitak, . and Kannika Chookietwattana, . The diversity of halotolerant and halophilic bacteria in the soil of the Nasinuan Secondary Forest in Maha Sarakham Thailand. pp. 166-176. ISSN 2672-7226

In the present study we investigated the diversity of halotolerant and halophilic bacteria in the soil of the Nasinuan Secondary Forest using the culturable approach. Halotolerant slightly halophilic and moderately halophilic bacteria were isolated from soil samples using the halobacteria medium containing 0 0.03 and 6 w/v sodium chloride respectively. In total 55 isolates were obtained. A decreasing trend of bacterial diversity was found with the increase in concentration of sodium chloride in the medium. From a comparison of cell and colony morphologies 16 isolates were selected for identification based on 16S rDNA sequence analysis. Of the 16 isolates 14 isolates were halotolerant bacteria and 2 isolates were slightly halophile. Many of them had an ability to grow in a wide range of NaCl (0-8 w/v). The 16 isolates belonged to 5 genera namely Bacillus Enterobacter Janibacter Rhodococcus and Staphylococcus. The dominant species belonged to the genus Bacillus. The phylogenetic analysis showed genetic diversity covering 5 clusters: Gram-negative rods; Gram-positive rods/cocci nonendospore-forming; Bacillus sensu stricto; Bacillus sensu lato; and Gram-positive cocci. This is the first comprehensive study of culturable halotolarant and halophilic bacterial diversity in a non-saline soil of Thailand.

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Abstract

In the present study we investigated the diversity of halotolerant and halophilic bacteria in the soil of the Nasinuan Secondary Forest using the culturable approach. Halotolerant slightly halophilic and moderately halophilic bacteria were isolated from soil samples using the halobacteria medium containing 0 0.03 and 6 w/v sodium chloride respectively. In total 55 isolates were obtained. A decreasing trend of bacterial diversity was found with the increase in concentration of sodium chloride in the medium. From a comparison of cell and colony morphologies 16 isolates were selected for identification based on 16S rDNA sequence analysis. Of the 16 isolates 14 isolates were halotolerant bacteria and 2 isolates were slightly halophile. Many of them had an ability to grow in a wide range of NaCl (0-8 w/v). The 16 isolates belonged to 5 genera namely Bacillus Enterobacter Janibacter Rhodococcus and Staphylococcus. The dominant species belonged to the genus Bacillus. The phylogenetic analysis showed genetic diversity covering 5 clusters: Gram-negative rods; Gram-positive rods/cocci nonendospore-forming; Bacillus sensu stricto; Bacillus sensu lato; and Gram-positive cocci. This is the first comprehensive study of culturable halotolarant and halophilic bacterial diversity in a non-saline soil of Thailand.

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Item Type:	Article
AGROVOC Term:	Bacteria
AGROVOC Term:	Bacterial flora
AGROVOC Term:	Secondary forests
AGROVOC Term:	Forest soils
AGROVOC Term:	Saline soils
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Bacillus
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Rhodococcus (bacteria)
AGROVOC Term:	Enterobacter
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9637

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