Detection, isolation and antibiogram of vibrio parahaemolyticus from frozen keropok lekor in Terengganu, Malaysia.

Adebisi, Naziahsalam Kehinde and Asmaliza Abg. Ghani and Tang, John Yew Huat (2023) Detection, isolation and antibiogram of vibrio parahaemolyticus from frozen keropok lekor in Terengganu, Malaysia. Journal of Agrobiotechnology (Malaysia), 14. pp. 32-43. ISSN 2180-1983

Vibrio parahaemolyticus is a Gram-negative bacterium that is found in the marine environment and causes acute gastroenteritis in humans. V. parahaemolyticus is an important foodborne pathogen found to contaminate fish and fish products. Thus, this study aims to detect and characterize V. Parahaemolyticus isolated from frozen keropok lekor in Terengganu. A total of 120 keropok lekor samples in Terengganu were examined for the presence of V. Parahaemolyticus using the conventional plating method on Thiosulfate-Citrate Bile-salt Sucrose (TCBS) agar and Multiplex Polymerase Chain Reaction (mPCR) assay for detection of toxR, tdh and trh gene. The V. Parahaemolyticus isolates were tested for antimicrobial susceptibility using the disc diffusion method against 12 types of antibiotics and the Multiple Antibiotic Resistance (MAR) index was determined. The prevalence of V. Parahaemolyticus in keropok lekor was 64.3% in Kuala Besut, 19% in Kuala Nerus and 0% in Setiu, respectively. All the isolates possess the trh gene but not the tdh gene. MAR index of V. Parahaemolyticus isolates was between 0.25 and 0.58. This study proved that the frozen keropok lekor were contaminated with multiple antibiotic resistant V. parahaemolyticus.

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Abstract

Vibrio parahaemolyticus is a Gram-negative bacterium that is found in the marine environment and causes acute gastroenteritis in humans. V. parahaemolyticus is an important foodborne pathogen found to contaminate fish and fish products. Thus, this study aims to detect and characterize V. Parahaemolyticus isolated from frozen keropok lekor in Terengganu. A total of 120 keropok lekor samples in Terengganu were examined for the presence of V. Parahaemolyticus using the conventional plating method on Thiosulfate-Citrate Bile-salt Sucrose (TCBS) agar and Multiplex Polymerase Chain Reaction (mPCR) assay for detection of toxR, tdh and trh gene. The V. Parahaemolyticus isolates were tested for antimicrobial susceptibility using the disc diffusion method against 12 types of antibiotics and the Multiple Antibiotic Resistance (MAR) index was determined. The prevalence of V. Parahaemolyticus in keropok lekor was 64.3% in Kuala Besut, 19% in Kuala Nerus and 0% in Setiu, respectively. All the isolates possess the trh gene but not the tdh gene. MAR index of V. Parahaemolyticus isolates was between 0.25 and 0.58. This study proved that the frozen keropok lekor were contaminated with multiple antibiotic resistant V. parahaemolyticus.

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Item Type:	Article
AGROVOC Term:	seafoods
AGROVOC Term:	Vibrio parahaemolyticus
AGROVOC Term:	sampling
AGROVOC Term:	microbiological analysis
AGROVOC Term:	frozen foods
AGROVOC Term:	frozen products
AGROVOC Term:	food safety
AGROVOC Term:	food quality
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	27 Aug 2024 15:26
Last Modified:	27 Aug 2024 15:26
URI:	http://webagris.upm.edu.my/id/eprint/965

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