Citation
Puvaneswari Puvanasundram, . and Chean Beryl Mei Yun, . and Jasmin Yaminudin, . and Murni Karim, . Evaluation of antagonism activity and control of Vibrio alginolyticus in Artemia culture using mixed probiotic. pp. 117-137. ISSN 1511-3701
Abstract
Supplementation with mixed probiotic in aquaculture has been proven to benefit the hosts as disease resistance tool. In this study a mixed probiotic which consisted of three isolated strains (Lysinibacillus fusiformis strain SPS11 A2 and Bacillus megaterium strain I24) was formulated for the in vitro assays against Vibrio alginolyticus and in vivo preliminary study towards Artemia nauplii. These strains showed antagonism activities against V. alginolyticus in in vitro assay. An increase in biofilm formation of this mixed probiotic was observed which indicated that the strains could work synergistically with each other to confer benefits to the hosts. Enrichment of Artemia nauplii with the formulated mixed probiotic was done to investigate its role in enhancing resistance against the V. alginolyticus. Artemia nauplii were cultured in two different concentrations of mixed probiotic (10� and 10� CFU mL�) and challenged via immersion method. The mixed probiotic at both concentrations resulted in significantly higher survival of Artemia compared to the challenged group with no probiont added (10� CFU mL� 65.00 0.00 and 10� CFU mL� 77.50 3.53 ). Significant reduction of Vibrio loads was observed in Artemia and its culture water supplemented with mixed probiotic at 10� CFU mL-1 whereas there was no reduction of Vibrio at 10� CFU mL�. This study suggests that the usage of formulated mixed probiotic at high concentration (10� CFU mL-1) as opposed to single-strain probiotic can confer protection against V. alginolyticus infection towards Artemia.
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Abstract
Supplementation with mixed probiotic in aquaculture has been proven to benefit the hosts as disease resistance tool. In this study a mixed probiotic which consisted of three isolated strains (Lysinibacillus fusiformis strain SPS11 A2 and Bacillus megaterium strain I24) was formulated for the in vitro assays against Vibrio alginolyticus and in vivo preliminary study towards Artemia nauplii. These strains showed antagonism activities against V. alginolyticus in in vitro assay. An increase in biofilm formation of this mixed probiotic was observed which indicated that the strains could work synergistically with each other to confer benefits to the hosts. Enrichment of Artemia nauplii with the formulated mixed probiotic was done to investigate its role in enhancing resistance against the V. alginolyticus. Artemia nauplii were cultured in two different concentrations of mixed probiotic (10� and 10� CFU mL�) and challenged via immersion method. The mixed probiotic at both concentrations resulted in significantly higher survival of Artemia compared to the challenged group with no probiont added (10� CFU mL� 65.00 0.00 and 10� CFU mL� 77.50 3.53 ). Significant reduction of Vibrio loads was observed in Artemia and its culture water supplemented with mixed probiotic at 10� CFU mL-1 whereas there was no reduction of Vibrio at 10� CFU mL�. This study suggests that the usage of formulated mixed probiotic at high concentration (10� CFU mL-1) as opposed to single-strain probiotic can confer protection against V. alginolyticus infection towards Artemia.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Artemia |
AGROVOC Term: | Prawns and shrimps |
AGROVOC Term: | Aquaculture |
AGROVOC Term: | Animal feeding |
AGROVOC Term: | Probiotics |
AGROVOC Term: | Feed supplements |
AGROVOC Term: | Feed additives |
AGROVOC Term: | Vibrio |
AGROVOC Term: | Antagonism |
AGROVOC Term: | Bacterial diseases |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:55 |
URI: | http://webagris.upm.edu.my/id/eprint/9665 |
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