Evaluation of total phenolic content and antioxidant activities from different type of extraction technique of Helianthus tuberosus

Nur Diyana Alyas, . and Fadzilah Puteh, . and Nurin Izzati Mohd Zulkifli, . and Noor Zafira Noor Hasnan, . Evaluation of total phenolic content and antioxidant activities from different type of extraction technique of Helianthus tuberosus. pp. 1-12. ISSN 2735-1084

This study investigated the difference of antioxidants activity from different extraction techniques of Jerusalem artichoke (Helianthus tuberosus). Three different extraction techniques involved were aqueous extraction high-pressure steam extraction and 70 ethanol extraction. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method. The antioxidant activities were analysed using ferric reducing antioxidant power (FRAP) and 2 2-diphenyl-1-picrylhydrazyl (DPPH). The high-pressure steam extract showed the highest total phenolic content and both FRAP and DPPH activities at 127.87 0.08 mg GAE/g 53.38 2.76 mg AAE/g and 79.92 0.26 mg AAE/g. Meanwhile the aqueous extract showed 2-fold higher of total phenolic content compared to ethanol extract at 65.73 9.44 and 30.61 0.00 mg GAE/g. Conversely antioxidant activities obtained from ethanol extracts (41.49 2.54 and 41.10 0.10 mg AAE/g.) were higher compared to aqueous extract (23.29 0.47 and 5.24 0.38 mg AAE/g.). This study suggests that different extraction methods influenced the findings of total phenolic content and antioxidant activities of the Jerusalem artichoke. Hence Jerusalem artichoke can be considered as a potential source of natural antioxidants.

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Abstract

This study investigated the difference of antioxidants activity from different extraction techniques of Jerusalem artichoke (Helianthus tuberosus). Three different extraction techniques involved were aqueous extraction high-pressure steam extraction and 70 ethanol extraction. Total phenolic content (TPC) was determined using the Folin-Ciocalteu method. The antioxidant activities were analysed using ferric reducing antioxidant power (FRAP) and 2 2-diphenyl-1-picrylhydrazyl (DPPH). The high-pressure steam extract showed the highest total phenolic content and both FRAP and DPPH activities at 127.87 0.08 mg GAE/g 53.38 2.76 mg AAE/g and 79.92 0.26 mg AAE/g. Meanwhile the aqueous extract showed 2-fold higher of total phenolic content compared to ethanol extract at 65.73 9.44 and 30.61 0.00 mg GAE/g. Conversely antioxidant activities obtained from ethanol extracts (41.49 2.54 and 41.10 0.10 mg AAE/g.) were higher compared to aqueous extract (23.29 0.47 and 5.24 0.38 mg AAE/g.). This study suggests that different extraction methods influenced the findings of total phenolic content and antioxidant activities of the Jerusalem artichoke. Hence Jerusalem artichoke can be considered as a potential source of natural antioxidants.

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Item Type:	Article
AGROVOC Term:	Helianthus
AGROVOC Term:	Helianthus tuberosus
AGROVOC Term:	Jerusalem artichokes
AGROVOC Term:	Extraction
AGROVOC Term:	Plant extracts
AGROVOC Term:	Techniques
AGROVOC Term:	Phenolic content
AGROVOC Term:	Antioxidants
AGROVOC Term:	Natural antioxidants
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9675

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