Palm tocotrienols cause cleavage of poly-(ADP)-ribose polymerase enzyme and down-regulation of Cyclooxygenase-2 protein level in human breast cancer cells

Kanga Rani Selvaduray, . and Radhika Loganathan, . and Kalanithi Nesaretnam, . and Ammu Kutty Radhakrishnan, . Palm tocotrienols cause cleavage of poly-(ADP)-ribose polymerase enzyme and down-regulation of Cyclooxygenase-2 protein level in human breast cancer cells. pp. 119-128. ISSN 1511-2780

Breast cancer is a leading cause of cancer-related deaths in women globally. The anti-cancer efects of various forms of vitamin E from palm oil tocotrienol-rich fraction (TRF): natural form of vitamin E complex in palm oil tocotrienol-enriched fraction (TEF) and major vitamin E homologues in palm oil: -tocopherol (Toc) and tocotrienols (T3) ( ´ or ) were tested on two human breast cancer cell lines MDA-MB-231 (triple negative) and MCF-7 (oestrogen-dependent). Chronic infammation plays a key role in tumourigenesis. Both cell lines used express high levels of poly-(ADP)-ribose polymerase-1 (PARP-1) and cyclooxygenase-2 (COX-2) which are key mediators of infammation. Tocotrienols exerted marked anti-proliferative by promoting apoptosis in both MDA-MB-231 and MCF-7 cells. In addition T3 also induced time-dependent inactivation of PARP-1 as well as inhibited expression of COX-2 in both MDA-MB-231 and MCF-7 cells. The rate of T3 uptake was found to be comparable to the anti-proliferative and apoptotic activities observed. In conclusion T3 induced marked anti-proliferative (p0.05) and pro-apoptotic (p0.05) efects which were most likely associated with PARP-1 inactivation and COX-2 down-regulation in these human breast cancer cells.

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Abstract

Breast cancer is a leading cause of cancer-related deaths in women globally. The anti-cancer efects of various forms of vitamin E from palm oil tocotrienol-rich fraction (TRF): natural form of vitamin E complex in palm oil tocotrienol-enriched fraction (TEF) and major vitamin E homologues in palm oil: -tocopherol (Toc) and tocotrienols (T3) ( ´ or ) were tested on two human breast cancer cell lines MDA-MB-231 (triple negative) and MCF-7 (oestrogen-dependent). Chronic infammation plays a key role in tumourigenesis. Both cell lines used express high levels of poly-(ADP)-ribose polymerase-1 (PARP-1) and cyclooxygenase-2 (COX-2) which are key mediators of infammation. Tocotrienols exerted marked anti-proliferative by promoting apoptosis in both MDA-MB-231 and MCF-7 cells. In addition T3 also induced time-dependent inactivation of PARP-1 as well as inhibited expression of COX-2 in both MDA-MB-231 and MCF-7 cells. The rate of T3 uptake was found to be comparable to the anti-proliferative and apoptotic activities observed. In conclusion T3 induced marked anti-proliferative (p0.05) and pro-apoptotic (p0.05) efects which were most likely associated with PARP-1 inactivation and COX-2 down-regulation in these human breast cancer cells.

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Item Type:	Article
AGROVOC Term:	Cancer (disease)
AGROVOC Term:	Breasts
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Experimental design
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Cells
AGROVOC Term:	Vitamin e
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9797

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