Citation
Damayanti Putri, . and Kurnia Nani, . and Rante Herlina, . and Ali Alimuddin, . and Ulfah Andi Asrini Nurani, . and Jumadi Oslan, . Diversity of endophytic actinomycetes producing indole-3- acetic acid and In vitro Evaluation of plant growth-promoting activity on Brassica oleracea L. pp. 275-292. ISSN 1511-3701
Abstract
The evaluation of endophytic actinomycetes diversity growth-promoting strain effects on cauliflower seeds germination and in vitro organogenesis are the objectives in this study. Moreover 15 strains from 125 isolates were determined to produce indole-3- acetic acid (IAA) where majority was obtained from roots (66.67) followed by from branches (26.67) and leaves (6.67). Specifically Jatropha sp. is a plant species with the most endophytic actinomycetes content compared to others. In addition all endophytic Streptomyces strains were screened based on IAA production ability in vitro on yeast extract“malt extract broth (YMG) broth medium. The results showed the tendency for one strain with code Streptomyces sp. KMR-1E to generate a maximum IAA isolate from Cinnamomun sp. plant. Furthermore the molecular taxonomy and phylogenetic analysis were recreated from 16S rRNA gene sequences which attributed the KMR-1E to genus Streptomyces. Meanwhile plant growth promotion was evaluated under in vitro condition. This exposed the individual ability to enhance the shoot and root length of cauliflowers. The untreated cultures with a strain free agar block was used as control.
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Abstract
The evaluation of endophytic actinomycetes diversity growth-promoting strain effects on cauliflower seeds germination and in vitro organogenesis are the objectives in this study. Moreover 15 strains from 125 isolates were determined to produce indole-3- acetic acid (IAA) where majority was obtained from roots (66.67) followed by from branches (26.67) and leaves (6.67). Specifically Jatropha sp. is a plant species with the most endophytic actinomycetes content compared to others. In addition all endophytic Streptomyces strains were screened based on IAA production ability in vitro on yeast extract“malt extract broth (YMG) broth medium. The results showed the tendency for one strain with code Streptomyces sp. KMR-1E to generate a maximum IAA isolate from Cinnamomun sp. plant. Furthermore the molecular taxonomy and phylogenetic analysis were recreated from 16S rRNA gene sequences which attributed the KMR-1E to genus Streptomyces. Meanwhile plant growth promotion was evaluated under in vitro condition. This exposed the individual ability to enhance the shoot and root length of cauliflowers. The untreated cultures with a strain free agar block was used as control.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Cauliflowers |
AGROVOC Term: | Brassica oleracea |
AGROVOC Term: | In vitro |
AGROVOC Term: | Organogenesis |
AGROVOC Term: | Sampling |
AGROVOC Term: | Statistical analysis |
AGROVOC Term: | Actinomycetes |
AGROVOC Term: | Indoleacetic acid |
AGROVOC Term: | Plant growth promoting bacteria |
AGROVOC Term: | Seed germination |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:55 |
URI: | http://webagris.upm.edu.my/id/eprint/9839 |
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